摘要
目的用酶标法（ELISA）和荧光定量PCR法（PCR）检测5岁以下儿童下呼吸道感染嗜肺军团菌（Lp）的感染情况。方法对300例5岁以下下呼吸道感染儿童用ELISA法检测血清Lp-IgM、IgG抗体、荧光定量PCR法测痰或咽拭子Lp-DNA。各组数据阳性率用卡方（x^2）检验，两种方法检测的一致性用配对计数资料的McNemar检验。结果ELISA法测抗体17例阳性，阳性率5．67％，IgM阳性10例，IgG阳性7例。其中男11例（阳性率5．76％），女6例（阳性率5．50％）。27d至1岁4例（阳性率2．53％），1-3岁7例（阳性率7.37％），3～5岁6例（阳性率12．77％）。冬春季7例（阳性率5．19％），夏秋季10例（阳性率6．06％）。来自城市11例（阳性率11．83％），农村6例（阳性率2．90％）。PCR法测DNA16例阳性，阳性率5．33％。其中男10例（阳性率5．24％），女6例（阳性率5．50％）。27d～1岁5例（阳性率3．16％），1～3岁6例（阳性率6．32％），3～5岁5例（阳性率10．64％）。冬春季3例（阳性率2．22％），夏秋季13例（阳性率7．88％）。来自城市9例（阳性率9．68％），农村7例（阳性率3．38％）。ELISA、PCR两种检测方法的配对计数资料McNemar检验Qm值0．0370，Pr值0．8474。结论5岁以下下呼吸道感染患儿有一定的嗜肺军团菌感染率。ELISA、PCR两种检测方法都显示不同年龄嗜肺军团菌感染阳性率随年龄增长而增加，感染者城区的阳性率明显高于农村。ELISA、PCR两种检测方法的一致性较好。
Objective To investigate the infection rate of Legionella pneumophila （LP） in children younger than 5 years with lower respiratory infections by ELISA and PCR. Method Serum LP-IgM and IgG were measured by ELISA, and LP DNA in sputum or throat swab were detected by PCR in 300 children less than 5 years with lower respiratory infections. The data were analyzed by chi-square test and the consistency of the two methods was analyzed by McNemar test. Results Serum antibody detected by ELISA was positive in 17 cases （5.67 % ）, including 10 positive of IgM and 7 positive of IgG. In these 17 cases, 11 were males and 6 were females with similar positive rates （5.76% vs 5.5%）. Four cases （2.53%） were positive in children aged from 27 days to 1 year, while 7 （7.37%） and 6 cases （12.77%） were positive in children aged 1 - 3 years and 3 - 5 years, respectively. Seven cases （5.19%） found in the winter and spring seasons and 10 cases （6.06%） in summer and autumn seasons. Eleven children （ 11.83 % ） were from urban area and only 6 （2.9 % ） from countryside. DNA in sputum or throat swab detected by PCR was positive in 16 cases （5.33%）, included 10 males and 6 females with similar positive rates （5.24% vs 5.5%）. Five cases （3.16%） were positive in children aged from 27 days to 1 year, while 6 （6.32%） and 5 cases （10.64%） were positive in children aged 1 - 3 years and 3 - 5 years, respectively. Three cases （2.22%） found in the winter and spring seasons and 13 cases （7.88%） in summer and autumn seasons. Nine children （9.68%） were from urban area and only 7 （3.38%） from countryside. McNemar test was used to compare the data of ELISA and PCR results with a Qm of 0.037 and a Pr of 0. 8474. Conclusions LP might contribute partly to the lower respiratory infections in children less than 5 years. The infection rate of LP increases with age increasing. Urban children have a higher infection rate than those living in countryside. Both methods have a good consistency
出处
《中华急诊医学杂志》
CAS
CSCD
2008年第5期517-520,共4页
Chinese Journal of Emergency Medicine
基金
浙江省科技厅项目（2004C33046）
