摘要
研究了高效液相色谱法（HPLC）测定生物柴油生产中副产物甘油的方法。生物柴油废弃物（主要含甘油）经稀释、调节pH至5．5，离心，所得下层液经微孔滤膜过滤，HPLC检测其甘油含量。最佳色谱条件：色谱柱：ZORBAX NH2（250mm×4．6mm，5μm）；检测器：示差检测器；柱温30℃：流动相：70％乙腈；流速1mL／min。甘油最低检测限为2mg／L，回收率为94．34％～99．43％，相对标准偏差为1．03％。与传统化学方法相比，高效液相色谱法快速简便，结果准确，重现性好。
The glycerol in the byproduct of biodiesel was determined by HPLC. The biodiesel byproduct containing glycerol was diluted and neutralized to pH 5.5 by H3 PO4, and then was centrifuged. The bottom layer（ crude glyceride）was microfiltrated,and then was analyzed by HPLC. The optimal conditions of HPLC were as follows ： the column of chromatogram ZORBAX NH2 （ 250 mm × 4.6 mm,5 μm）, mobile phase 70% acetonitrile,flow rate 1.0 mL/min, column temperature 30 ℃, differential refractive index detector. The lowest of measuring limit was 2 mg/L,the recovery of the method was 94. 34% -99.43% in the linear range of content, and the relative standard deviation was 1.03%. Compared with traditional chemical method, this method is reliable, simple, quick and economic, and it seems to be an ideal method for determining the glycerol in the byproduct of biodiesel.
出处
《中国油脂》
CAS
CSCD
北大核心
2008年第3期74-77,共4页
China Oils and Fats
