摘要
根据小反刍兽疫病毒疫苗株Nigeria75/1的全基因序列,通过PCR方法,将N基因亚克隆入pBAD/TOPO表达载体,构建了原核表达质粒pBAD-PPRN。该重组质粒转化至大肠埃希菌TOP10中,经L-Arabinose诱导,SDS-PAGE和Western blot检测,表达的重组N蛋白分子质量与预期的73.6ku一致,为以小反刍兽疫N蛋白为抗原的诊断试剂盒研制奠定了基础。
The ORF sequence of gene N encoding Peste des petits ruminants virus neucleoprotein was synthesized according to the Nigeria 75/1 strain completely sequence. ORF sequence was cloned into pBAD/ TOPO vector and transformed into host Escherichia coli TOP10 competent cells and then induced by different concentration L-Arabinose. SDS-PAGE and Western-blot analysis indicated that the protein possessed antigenic epitopes of neucleoprotein. The result provided foundation for developing a diagnosis kit of Peste des petits.
出处
《动物医学进展》
CSCD
2008年第5期1-3,共3页
Progress In Veterinary Medicine
基金
国家质检总局科技计划项目(2007IK025)
关键词
小反刍兽疫
N基因
原核表达
Peste des petits ruminants
N gene
prokaryotic expression
