摘要
为解决氨基酸发酵工业中的噬菌体污染问题,对cglI基因复合体在钝齿棒杆菌中的功能活性表达进行研究。通过PCR从谷氨酸棒杆菌基因组扩增cglI基因复合体,构建重组质粒pJL23-cglI,转化钝齿棒杆菌T6-13后得到重组菌株。定性和定量检测重组菌株的噬菌体抗性。实验结果表明,携带cglI基因复合体的重组钝齿棒杆菌显示了明显的抗噬菌体功能活性和较广的抗噬菌体谱,进而证实了cglI基因复合体用于构建钝齿棒杆菌抗噬菌体菌株的可行性,为解决氨基酸发酵生产中的噬菌体污染问题提供了一种有效方法。
In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PeR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cg/I into C. crenatum. Results showed that the recombinant strains possessed strong phage- resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contamination in amino acid fermentation industry.
出处
《生物工程学报》
CAS
CSCD
北大核心
2008年第5期760-765,共6页
Chinese Journal of Biotechnology
基金
辽宁省科技厅计划项目(No.2004205004)资助~~
关键词
限制修饰系统
cglI基因复合体
钝齿棒杆菌
噬菌体
基因工程菌
restriction and modification system, cgll gene complex, Corynebacterium crenatum, bacteriophage, genetic engineered strain
