摘要
自江苏、山东、安徽3省不同地区的表观健康鹅群采集泄殖腔棉拭子样品,分离、鉴定新城疫病毒(NDV),研究其生物学特性和分子流行病学特征,结果从1 108份样品中分离到11株NDV。依据鸡胚平均死亡时间(MDT)及融合蛋白(F)裂解位点氨基酸序列,判定其中6株病毒为NDV弱毒株,3株病毒为NDV中等毒力株,2株病毒为NDV强毒株。对F基因的序列测定及分析表明,6个弱毒株与La Sota株高度同源,3个中等毒力株与Texas GB株高度同源,2个强毒株则与1997年以来流行的对鹅具高度致病性的NDV有较高的同源性,只是其F蛋白信号肽序列及另外3个特征性位点的氨基酸显著不同。
Waterfowls are considered to be the natural reservoir for certain viral pathogens including avian influenza virus and Newcastle disease virus（NDV）.In the present investigation,we performed isolation and characterization of NDV from apparently healthy domestic geese.Eleven isolates were obtained,of which 6 were considered to be lentogenic based on the MDT and ICPI values and the characteristic motif 112 G-R-Q-G-R-L 117 at the cleavage site of fusion（F） proteins,3 were considered mesogenic and the remaining 2 were determined as velogenic.Phylogenetic analysis revealed that the lentogenic isolates and 3 mesogenic ones are highly homologous to the reference strains La Sota and Texas GB,respectively,whereas the two velogenic viruses are closely related to those caused clinical diseases in geese since the late 1990s.However,none of the 11 isolates is identical or closely related to those geese pathogenic ND viruses at the amino acid level.Our results showed that both lentogenic and mesogenic and velogenic ND viruses are present in apparently healthy domestic geese flocks,necessitating a stricter biosecurity practice in poultry industry.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2008年第3期239-242,共4页
Chinese Journal of Veterinary Science
基金
江苏省青年创新人才基金资助项目(BK2003403)
江苏省教育厅自然科学基金资助项目(03KJB230158)
扬州大学高层次人才启动基金资助项目
关键词
鹅
新城疫病毒
融合蛋白
生物学特性
geese
Newcastle disease virus
F protein
biological characterization
