摘要
目的:比较高增殖潜能内皮祖细胞(HPP-EPCs)和循环成血管细胞(CACs)两种内皮祖细胞的成血管能力。方法:利用基质胶上毛细血管样结构的形成实验比较两种细胞的体外成血管能力;利用免疫缺陷的无胸腺裸鼠建立后肢缺血模型评价两种细胞移植后体内促进新生血管形成的能力;并利用荧光标记示踪的方法研究移植的内皮祖细胞能否整合入新生的毛细血管中。结果:HPP-EPCs形成的毛细血管样结构数明显高于CACs(28.6±15.8vs4.7±3.4,P<0.01)。内皮祖细胞移植能增加肢体的保留比例,减少肢体脱失率,促进缺血肢体的血流恢复,而且HPP-EPCs移植组的增加程度较CACs移植组的为高。HPP-EPCs和CACs移植组的毛细血管密度均高于对照组(P<0.001),且HPP-EPCs高于CACs移植组(P<0.01)。结论:HPP-EPCs较CACs具有更强的成血管能力。
Objective：To compare the in vitro angiogenic potential between two types of endothelial progenitor cells （EPCs）, the high proliferative potential-endothelial progenitor cells （HPP-EPCs） and circulating angiogenic cells （CACs）. Methods： Matrigel capillary-like structure forming experiments were performed to evaluate the angiagenic potential in vitro. Ischemia models were produced in immune-deficient athymic nude mice, which were then used to evaluate the angiogenic effects between HPP-EPCs and CACs in terms of three aspects： limb salvage, blood flow and capillary density. The incorporation of DiI-labelled EPCs into newly formed capillaries was confirmed under a confocal microscope. Results： More capillary-like structures was found with HPP-EPCs than with CACs （28.6± 15.8 vs 4.7 ± 3.4, P 〈0.01 ）. The results in animal models showed that EPC transplantation led to increased limb salvage, reduced limb loss and promoted blood flow to the ischemic limbs, with greater improvement in HPP-EPCs group compared with CACs group. Higher capillary density was noted in EPC-transplanted groups, HPP-EPC group in particular, as compared with the controls （P 〈 0.01 ）. Under confocal microscopy, DiI-labelled EPCs were observed to have been incorporated into newly formed capillaries. Conclusion： HPP-EPCs showed stronger angiogenic potentials than CACs, both in vitro and in vivo.
出处
《广州医学院学报》
2007年第5期15-19,共5页
Academic Journal of Guangzhou Medical College
基金
国家863项目基金(2003AA205181)
国家973项目基金(00CB51010)
国家自然科学基金重点项目(30030070)
中国博士后基金(20070420762)
湖南省自然科学基金(07JJ5034)
广州市医药卫生科技项目(2007-YB-003)
关键词
内皮祖细胞
血管形成
缺血
细胞移植
endothelial progenitor cells
angiogenesis
ischemia
cell transplantation
