摘要
建立阳春砂仁含量测定方法.采用气相色谱（GC）法测定阳春砂仁中乙酸龙脑酯的含量,采用高效液相色谱（HPLC）法测定阳春砂仁中槲皮苷的含量.乙酸龙脑酯进样量在0.1271～2.5424μg（r=0.99908）范围内与峰面积呈良好的线性关系,平均回收率为97.95%,相对标准偏差为2.08%（n=6）;槲皮苷进样量在0.006～0.013μg（r=0.99956）范围内与峰面积呈良好的线性关系,平均回收率为103.73%,相对标准偏差为0.80%（n=6）.本方法简便,快速准确,可作为阳春砂仁含量测定质量控制方法.
To establish methods for the determination of the quality of Amomum villosum. GC was adopted for the determination of bornyl acetae in A momum villosum, HPLC was adopted for the determination of quercitroside in Amomum villosum. There was a good liner relationships between the peak areas and the sample contents within the ranges of 0. 127 1-2. 542 4 μg（ r = 0. 999 08） and 0. 006-0. 013 μg（ r = 0. 999 56） for bornyl acetae and quercitroside respectively. The average recovery rates of bornyl acetae and quercitroside were 97.95 % （ n = 6, RSD = 2.08 % ） and 103.73 % （ n = 6, RSD = 0.80 % ）, respectively. The methods are simple, rapid and accurate and can be used for quality control of A momum villosum.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期286-290,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
云南省科技厅中药现代化(云南)基地专项项目资助(2002ZY-5)
