摘要
从南极深海底泥中分离到一株中度嗜盐菌NJS-2,经过16S rDNA(已提交GenBank,其登录号为DQ789389)序列分析、形态学和生理生化特征分析,该菌株初步鉴定为色盐杆菌属(Chromohalobacter).以其16S rDNA序列相似性为基础构建了包括8种相关种属在内的系统发育树.同源性分析表明,NJS-2与Chromohalobacter salexigens的16S rDNA相似性达到99%以上,应归属于色盐杆菌属,命名该菌株为Chromohalobactersp.NJS-2.研究了菌株NJS-2的相容性溶质四氢嘧啶(Ectoine)的累积及累积条件.研究发现,30℃条件下,在含2 mol/L NaCl的培养基中培养48 h,可最大诱导生成Ectoine 300 mg/L.反复渗透性休克试验表明:NJS-2在低渗条件下能够快速将细胞内的Ectoine分泌到细胞外,而在高渗环境中能够较快地重新合成.
A moderately halophilic bacterium（designated strain NJS-2）was isolated from Antarctica deep-sea sediment. Based on the analysis of 16S rDNA（GenBank accession number DQ789389）, morphology, physiological and biochemical characteristics, strain NJS-2 was identified as Chromohalobacter sp.. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relative bacteria species. According to the phylogenetic tree,the strain was more relative to Chromohalobacter salexigens, the sequence homology was more than 99%, so NJS-2 should belong to Chromohalobacter sp. Synthesis capability and synthesis condition Ectoine（a complemental solute of osmotic pressure） from the NJS-2 strain were studied. When NJS-2 was cultivated in the bouillon culture-medium including 2 mol/L NaCl for 48 h at 30℃ ,the Ectoine synthesis amount was 280.4 mg/L.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期402-407,共6页
Journal of Xiamen University：Natural Science
基金
国家大洋专项(DY105-04-02-06)资助
