摘要
采用鸡毛水解液和头发水解液代替常规黄豆粉培养基中的氮源进行发酵培养苏云金杆菌,在发酵过程中测定总菌数,芽孢数和pH值等指标,并与常规黄豆粉培养基进行了比较。结果表明,用鸡毛水解液和头发水解液培养的B.t546在48h总菌数达到5.0×108个·mL-1左右,芽孢数达到1.0×108～2.0×108个·mL-1左右,效果略低于常规黄豆粉培养基(48h总菌数4.8×109个·mL-1,芽孢数2.6×109个·mL-1),但可以达到工业生产水平(芽孢108个·mL-1);而且镜检结果表明,毛发水解液培养的B.t546在18h左右就出现芽孢和伴孢晶体,比常规黄豆粉培养基出现时间要早6h左右。以棉铃虫为供试虫进行了室内毒力测定,利用毛发水解液生产的苏云金芽孢杆菌比常规黄豆粉培养基生产的苏云金芽孢杆菌杀虫效果略高。因此,利用毛发等废弃物生产生物农药以降低其生产成本是一个值得推广的废物资源化方法。
Bacillus thringiensis 546 was fermented by using hair hydrolyte as N sources to replace soybean meal medium in this study. Viable cell, sporulation and pH were evaluated by colony fermentation approach in the hair hydrolyte medium and conventional soybean meal medium. The results showed that the growth and sporulation of B.t depended, to a great extent, on the composition of medium especially for different N sources. The rate of sporulation in the hydrolyte medium was 6 hours earlier than that in the conventional soybean meal medium. The number of viable cells in the hair hydrolyte medium with 5.0×10^8 cells· mL^-1 were a little lower than in the soybean meal medium with 4.8×10^9 cells ·mL^-1 at 48 hours. Likewise, it was found that the hair hydrolyte medium produced 1.0×10^8-2.0×10^8 cells·mL^-1 of B.t spores, which was similar to that obtained from the conventional soybean meal medium at 2.6× 10^9 cells· mL^-1. Furthermore, higher entomotoxicity using the Helicoverpa armigera as a worm of bioassay was observed in the hair hydrolyte mediums compared to the normal soybean meal medium.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第3期1248-1253,共6页
Journal of Agro-Environment Science
关键词
毛发
水解液
发酵
苏云金杆菌
毒力
hair
hydrolyte
fermentation
Bacillus thringiensis
entomotoxicity
