摘要
电场作用下根际土壤微生物群落的变化与利用电场强化植物修复效率密切相关。利用改进的PCR-DGGE方法研究了不同电场条件下根际土壤微生物群落多样性和相似性的变化,分析了直流电场对土壤微生物群落的影响机理。结果表明,电场对根际土壤微生物群落的影响与电场条件有关,合适的电场条件有助于增加土壤微生物群落的多样性,但是电场形式、强度和施加方式不当则会使土壤微生物群落的多样性和结构受到明显影响。电场作用下土壤性质的变化、电场对土壤微生物的迁移作用和致死效应,以及微生物对环境压力的生理响应等是电场影响土壤微生物群落的主要机制。为了避免电场对根际土壤微生物的不利影响,利用电场强化植物修复时需要采用合适的电场条件。
The change of rhizospheric soil microbial communities under electric fields influences the efficacy of using direct current fields to accelerate the process of phytoremediation. In this study, the diversity and similarity of rhizospheric soil microbial communities under different electric fields were investigated through an improved PCR-DGGE analysis and the mechanisms by which electric fields influence soil microbial communities were further analyzed. The results showed that the diversity and structure of rhizospheric soil microbial communities were greatly affected by the type and strength of electric fields and the application method; their diversity increased to a certain content under a proper electric field （in this study, that is, uniform electric field in a strength of 1.0 V.cm^-1 with a reversal time interval of 10 min）, while their diversity was reduced obviously and their structure was changed greatly otherwise. The induced change in soil properties such as soil pH, the mobilization of soil bacterial cells by electric fields, the lethal effect of electric fields with over high strength, and the induced alteration of gene regulation patterns response physiologically to environmental stress and change might be the main mechanisms influencing the soil microbial communities in electric fields. In order to avoid the detrimental effects of electric fields applied on rhizospheric microbial communities, it is necessary to adopt an appropriate electric field when using electric fields to accelerate the process of phytoremediation.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第3期920-925,共6页
Journal of Agro-Environment Science
基金
上海市自然科学基金项目(06ZR14146)
