摘要
目的:观察anti-miR-155反义寡核苷酸(AMOs)对肺鳞癌H157细胞增殖的影响。方法:将H157细胞分为对照组和AMOs处理组,AMOs处理组采用AMOs抑制H157细胞内miR-155的活性,液闪计数仪测定[3H]-TdR掺入量,MTT法测定细胞增殖抑制率,流式细胞仪测定细胞周期。结果:与对照组相比,AMOs显著减少H157细胞[3H]-TdR掺入量(P<0.01),随着浓度从10 nmol/L逐渐增加至于100 nmol/L,H157细胞[3H]-TdR掺入量亦随之减少(P<0.01);AMOs显著抑制H157细胞的增殖(P<0.01),使G0/G1细胞比例显著增加,G2/M期细胞比例显著减少(P<0.01)。结论:AMOs通过抑制H157细胞内高水平表达miR-155的活性,显著抑制H157细胞的增殖。
Objective： To determine the effect of anti - miR - 155 oligonucleotides （AMOs） on proliferation of human lung squamous cell carcinoma cell line H157. Methods：Cultured H157 cells were divided into two groups： control group and AMOs group, In AMOs group, AMOs were used to inhibit the activity of miR - 155. [^3H]-TdR incorporation was detected by liquid scintillation counting, MTT assay was used to examine the inhibition percentage of AMOs to the proliferation of H157 cells and cell cycle was measured by flow cytometry. Results： AMOs decreased significantly the [^3H]-TdR incorporation in a dose - dependent manner （P〈0. 01）. With the increment of concentration of AMOs, the inhibition percentage increased significantly （P〈0. 01）. AMOs decreased significantly the cell ratios of G2/M phase and S phase, simultaneously increased the cell ratio of G0/G1 phase （P〈0.01）. Conclusion： AMOs inhibit the activity of miR - 155 expressing highly in H157 cells; as a result, inhibit significantly the proliferation of H157 cells.
出处
《西南国防医药》
CAS
2008年第3期322-324,共3页
Medical Journal of National Defending Forces in Southwest China
基金
国家自然科学基金项目(30672076)
国家重点基础研究发展"863"计划资助(2007AA02Z129)
