摘要
应用支气管肺泡灌洗法从诱导成功的卡氏肺孢子虫（ＰＣ）肺炎大鼠中分离ＰＣ虫体后，经抗大鼠白细胞共同抗原单克隆抗体（ＡｎｔｉＣＬＡＡｂ）处理使ＰＣ高度纯化，然后观察ＰＣ与小鼠和大鼠的巨噬细胞在体外的相互作用。结果显示，腹腔巨噬细胞（ＰＭφ）和肺泡巨噬细胞（ＡＭφ）能吞噬并降解ＰＣ虫体，ＰＭφ和ＡＭφ与ＰＣ相互作用后每１００个Ｍφ吞噬成熟ＰＣ包囊数分别为５．８０±０．７０和４．７０±０．５１（Ｐ＞０．０５，作用３０ｍｉｎ时）、１１．３３±０．７３和１１．５０±０．７６（Ｐ＞０．０５，作用６０ｍｉｎ时）。经过地塞米松预处理与未经处理的ＡＭφ，每１００个吞噬成熟ＰＣ包囊数分别为４．７５±０．３６和５．４２±０．２９（３０ｍｉｎ，Ｐ＞０．０５）、１０．０８±０．３９和１２．２５±０．２８（６０ｍｉｎ，Ｐ＜０．０５）。这说明糖皮质激素可抑制ＡＭφ的吞噬功能，推测糖皮质激素诱导大鼠产生ＰＣ肺炎可能与其抑制ＡＭφ的吞噬功能有关。
The Pneumocystis carinii (PC) were isolated through bronchial alveolar lavage from PC pneumonia rats prepared by induction with prednisolone, and it was treated and purified with antiratcommon leukocyte antigen monocolonal antibody. The ingesting ability of macrophage for PC and the effect of glucocorticoid were studied in vitro. The results showed that the PC could be ingested and degraded by peritoneal macrophage (PM φ) and alveolar macrophage (AM φ). The difference of uptake of mature cyst forms (MCFs) of PC between PM φ and AM φ was not significant(P>0.05). The ingesting ability of AM φ for PC in dexamethasone pretreatment group was inhibited after 60 min of phagocytosis in comparison with nonpretreatment group (the uptake of MCFs of PC:10.08±0.39/100M φ vs 12.25±0.28/100M φ,P<0.05). The results suggested that the induction of PC pneumonia in rats with the glucocorticoid may be related to the inhibition of macrophage ingestion ability, and that macrophage may play an important role in the protection of the pathogenesis of PC.
出处
《上海实验动物科学》
1997年第4期203-206,共4页
Shanghai Laboratory Animal Science
基金
上海市卫生局基金
上海医科大学基金
关键词
巨噬细胞
吞噬
卡氏肺孢子虫
感染
动物实验
Macrophage Ingestion Pneumocystis carinii In vitro Glucocorticoid Rat Mouse
