摘要
盆栽条件下播种黄瓜Cucumis sativus同时接种丛枝菌根真菌Glomus etunicatum,4周后对接种处理和对照黄瓜苗分别浇灌Fusairum oxysporum f.sp.cucumerinum分生孢子悬液,2周后测定幼苗生物量、根内丙二醛、可溶性糖与游离脯氨酸含量及根围真菌和细菌数量。结果表明:接种Glomus etunicatum根系干重增加了9.3%,提高了根内可溶性糖和游离脯氨酸含量,显著减少了根围真菌数量,降低了黄瓜枯萎病的发病率和病情指数。而不接种Glomus etunicatum的黄瓜苗根系干重减少了28.0%。研究认为AM真菌Glomus etunicatum对黄瓜枯萎病具有一定的生防价值。
Pot experiment was conducted to explore whether nursery inoculation of cucumber with Glomus etunicatum could alleviate fusarium wilt caused by Fusarium oxysporum f. sp, cucumerinum, Four-week-old seedlings inoculated with Glomus etunicatum were infected with F. oxysporum f. sp. cucumerinum by pouring conidial suspension. Biomass, contents of malonaldehyde （MDA）, soluble sugar and free proline in roots, as well as the quantity of bacteria and fungi in rhizosphere were determined. The results indicated that the root dry weight of seedlings preinoculated with Glomus etunicatum increased by 9.3%; contents of soluble sugar and free proline in roots increased, and the quantity of fungi in rhizosphere decreased significantly. The disease incidence and disease index of Fusarium wilt were reduced. On the contrary, root dry weight of seedlings without inoculation with Glomus etunicatum was reduced by 28.0%. It is concluded that Glomus etunicatum is beneficial to biocontrol of Fusarium wilt of cucumber seedlings.
出处
《菌物学报》
CAS
CSCD
北大核心
2008年第3期395-404,共10页
Mycosystema
基金
黑龙江省教育厅科学技术研究面上项目(No.11511299)
黑龙江大学校青年基金(No.QL200530)
黑龙江大学博士启动金(No.200504)
关键词
生物量
可溶性糖
游离脯氨酸
根际细菌
根际真菌
biomass, soluble sugar, free proline, bacteria in rhizosphere, fungi in rhizosphere
