摘要
在静止条件下，用分光光度法对３个儿茶酚胺底物的漆酶催化氧化行为进行了考察．ＥＳＲ结果证明，这些底物在弱碱性条件下的漆酶催化反应经历了半醌自由基阴离子的阶段，Ｍｇ２＋离子的存在，提高了邻苯半醌自由基的动力学稳定性．结果还表明，儿茶酚胺底物的漆酶催化氧化产物，结构十分复杂，用二苯乙二胺只能捕获到少量对位羟基化的邻醌．
:The laccasecatalyzed oxidation of three catecholamine were investigated spectrophotometrically under pH=6.0～8.0 conditions.The result of ESR demonstrated that the laccasecatalyzed reaction of these substrates underwent the stage of the proton transportation and one electron oxidation under condition of weak basicity.The studies show that the products of laccasecatalyzed oxidation of these substrates were very complex,and a little of hydroxy Oquinone can be captured by DPE.
出处
《江西师范大学学报（自然科学版）》
CAS
1997年第4期342-346,共5页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
国家自然科学基金
关键词
漆树
漆酶
儿茶酚胺
氧化
催化
中间体
:Rhus vernicifera laccase
catecholamine
enzymatic oxidation
semiquinone radical
spectrophotometry
