摘要
运用免疫组化技术研究内毒素对肝细胞凋亡及Bcl-2表达的影响。48只SD大鼠随机分成两组,Ⅰ组为对照组(静脉注射生理盐水),Ⅱ组为内毒素组(静脉注射内毒素5mg/kg)。分别在注射后3h、4h、8h、12h每组各迫杀6只,用流式细胞术检测肝细胞的凋亡及免疫组化技术观察内毒素对肝细胞Bcl-2表达的影响。结果显示,Ⅱ组凋亡肝细胞百分比明显高于Ⅰ组(P<0.01);Ⅱ组呈上升趋势;Ⅰ组Bcl-2的积分光密度明显高于Ⅱ组(P<0.01),且Ⅱ组一直呈下降趋势。结果表明,在体内内毒素可诱导肝细胞凋亡和抑制Bcl-2的表达。
To study on the effect of endotoxin on the apoptosis and the expression of Bcl-2 in the liver. Forty-eight Wistar rats were randomly divided into two groups： the normal controls group （group Ⅰ ） and ET treatment group （group Ⅱ ）, Aftert three, four, eight and twelve hours liver samples were taken, Compared with control groups, the ET groups hepatocyte percentage of apoptosis significantly increased （P 〈 0.01）, And the immunohistochemical examination with Bcl-2 of ET groups exhibited that the Bcl-2 decrease markedly （P〈0.01） compared with the control groups. These results indicated that ET could stimulate apoptosis of hepatocyte and up-regulate the Bcl-2 gene expression in the liver. And the up-regulate the Bcl-2 gene can result in the reducing the apoptosis of hepatocyte.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2008年第5期359-362,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家自然科学基金资助项目(30560113)
