摘要
目的：制备将药物治疗与热疗相结合的靶向抗癌药物新剂型-司莫司汀磁性脂质体。方法：用改良化学沉淀法制备Fe3O4纳米磁性粒子，运用透射电镜和PE热分析系统进行表征，在高频交变磁场下进行体外加热试验。采用反相蒸发超声法加高速搅拌制备纳米司莫司汀磁性脂质体，用透射电镜、图像分析系统和能谱仪对其进行表征，HPLC法检验脂质体中司莫司汀的包封率。结果：Fe3O4纳米磁性粒子近似球形，粒径10～40nm。居里温度在120℃～140℃之间,其磁流体在高频交变磁场下可升温至35℃～47℃并保持恒定。以此磁性材料为载体制成的司莫司汀磁性脂质体的平均粒径为184．5nm，其中含有Fe3O4成份，药物包封率达到73．72％。结论：Fe3O4纳米粒子是制备医用磁性脂质体的良好载体，采用反相蒸发超声法加高速搅拌可制备纳米级Fe3O4磁性脂质体。
Destination： To prepare a target anticancer drug which combine the medical treatment and thermotherapy treatment-Semustine Magnetic Liposome. Method： To prepare the Fe3O4 magnetic nanoparticle with improved chemical precipitation method, to carry out the exosyndrome with TEM and PE TAS （thermal analysis system）, to take vitro heating test under the high-frequency atternation magnetic field. To adopt antiphase evaporation transonic method and high speed agitation method to prepare Semustine Magnetic Liposome, to carry out the exosyndrome with TEM, image analysis system and energy spectrometer, to test the envelopment rate of semustine in the liposome. Result： Fe3O4 magnetic nanoparticle is similar to a globe, the particle diameter is 10-40nm. Curie temperature is between 120℃ and 140℃. The magnetic fluid of it can be heated to 35℃ to 47℃ and to keep constancy under the high-frequency atternation magnetic field. Use the magnetic material as carrier to prepare Semustine Magnetic Liposome, the average particle diameter of it is 184.5 nm. It contains the chemical reconstituent of Fe3 O4, the drug envelopment rate is 73.72 %. Conclusion： The Fe3O4 magnetic nanoparticle is a good carrier to prepare medical magnetic liposome. The adoption of antiphase evaporation transonic method and high speed agitation method can prepare nanometer Fe3O4 magnetic liposome.
出处
《科技通报》
2008年第3期406-410,共5页
Bulletin of Science and Technology
基金
国家863项目(2007AA022008)
