摘要
目的:寻找加杨叶粗提液中的总黄酮的有效方法。方法:利用双水相体系萃取分离、紫外分光光度法直接测定。结果:萃取分离加杨叶总黄酮的最佳双水相体系是25%PEG 400与12%(NH_4)_2SO_4,最佳萃取条件为:pH=9,NaCl的添加量为3%,粗提液3 mL,温度25℃。结论:该方法的相对标准偏差(RSD)(?)0.28%(n=5),具有良好的精密度和选择性,为黄酮类化合物萃取分离的一种有效方法。
Objective： To find an effective method for extraction and purification of total flavone of Populus Canadensis Moench. leaves, Methods： In the experiment,extraction and content determination of flavone of Populus Canadensis Moench.leaves were carried out simultaneously by using aqueous two-phase extraction partitioning and UV-spectrophotometry method. Results： This aqueous two-phase system was prepared by dissolving suitable amounts of polyethylene glycol （PEG）and （NH4）2SO4 in aqueous solution,Flavone of Populus Canadensis Moench. leaves were recovered with optimum high yield and high concentration in the upper polymer-rich phase when 25 % PEG 400 and 12%（NH4 ）2SO4 were applied.The optimum extraction conditions were pH value 9, NaCI3 %, temperature 25 ℃ and leaching solution volume 3 mL. The precision （RSD）was 0.28% （n=5）. Conclusion： The method is proved to be good in reproducibility, simple and convenient in operation, and it is effective to extract flavone.
出处
《现代生物医学进展》
CAS
2008年第5期854-857,共4页
Progress in Modern Biomedicine
基金
北京林业大学研究生自选课题资助基金(06jj127)
关键词
双水相体系
加杨叶总黄酮
紫外分光光度法
Aqueous two-phase system
Total flavone of Populus Canadensis Moench.leaves
UV-spectrophotometry
