摘要
目的通过观察动物模型脑的钙蛋白酶(Calpain)Ⅰ表达的变化,探讨急性播散性脑脊髓炎的发病机制。方法①利用雌性Wistar大鼠建立自身免疫性脑脊髓炎(EAE)动物模型;②通过免疫组化染色动态观察EAE大鼠不同时段各组鼠脑内CalpainⅠ的分布及表达的变化情况。结果CalpainⅠ在发病后7d达高峰,且染色反应增强,与正常对照组相比有统计学意义(P<0.05)。结论EAE模型建立方法稳定、可靠,成功率高。CalpainⅠ在正常大鼠的中枢神经系统中广泛存在。EAE发病过程中,CalpainⅠ在鼠脑表达增高。
Objective To investigate the expression of Calpain Ⅰ in different periods during experimental auto-immune encephalomyelitis（EAE） in Wistar rats′ brain. Method 1. To establish EAE model using female Wistar rats; 2. Using immunohistochemical staining method, dynamically observe the distribution and changes of expression in different periods in EAE rats′ brain. Results Calpain Ⅰ reached peak at 7 days after onset,and staining was increased,which was statistically significant compared with the normal control group （P〈0.05）. Conclusion The method of establishing EAE model is stable, reliable, and with high success rate. Calpain I exists widely in the CNS of normal rats. The expression of Calpain Ⅰ is increased during the pathogenesis of EAE in the rat brain.
关键词
实验性自身免疫性脑脊髓炎
钙蛋白酶
中枢神经系统
Experimental auto-immune encephalomyelitis （ EAE ）
Calpain
Central nervous system（CNS）
