摘要
目的:测定清热解毒注射液中黄芩苷的含量。方法:采用高效液相色谱法,测定条件为:色谱柱为AgelaODS(5um,4.6×200mm),流动相:甲醇-0.2%磷酸溶液(47:53);检测波长:276nm;流速:1.0ml/min;柱温:30℃。结果:木犀草苷在50.03-500.3μg/ml浓度范围内线性关系良好,r=0.9994,平均回收率为99.5(RSD=0.31%,n=6)。结论:该方法稳定、可靠、专属性强,可用于清热解毒注射液中黄芩苷的含量测定。
The content of baicalin in qingrejiedu injection was determined by HPLC method. The test conditions as follows： a akasil ODS column by methanol and 0.2 % phosphoric acid, the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, and the temperature of the column 30 12. The results showed that the linear range of baicalin was from 50.03-500.3μg/ml with r was 0. 9994 and the average recovery was 99.6 % （RSD =0.31%, n=6）. This method was simple, reliable and provided a scientific basis for the quality controlling of qingrejiedu injection.
出处
《广州化工》
CAS
2008年第2期50-51,共2页
GuangZhou Chemical Industry
