摘要
用反相高效液相法在Waters Symmetry C18柱(5μm,4.6 mm×150 mm i.d)上测定八角茴香果实中的莽草酸.工作参数为:流速0.5 mL/min;进样体积2μL;检测波长220 nm;柱温30℃±1℃;流动相0.01 mol/L K2HPO4磷酸盐缓冲液(pH值为3,用磷酸调配)和体积分数3%甲醇.平均回收率为97.76%,RSD为1.937%(n=5).
OBJECTIVE To establish a RP - HPLC method for the determination of Shikimic Acid in Illicium verum Hook. f. METHODS In RP - HPLC analysis, a Waters Symmetry C 18 column （5μn, 4.6 mm × 150 mm, i. d. ） was selected as separation column at 30 ℃ ± 1 ℃ .The mobile phase consisted of 0.01 mol/L K2HPO4 phosphate buffer （pH = 3,I =0.17 mol/L） and 3% methanol.The flow rate was 0.5 mL/min and the wavelength of measurement was 220 nm.The average recovery was 97.76% with RSD of 1.937% （n = 5）. CONCLUSION The method was proved to be quick, simple, and highly sensitive and can be useful in Shikimic Acid medicines quality control.
出处
《吉首大学学报（自然科学版）》
CAS
2008年第1期104-106,共3页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省科技厅重点资助项目(06FJ4046)
