摘要
目的观察山莨菪碱对离体大鼠腹主动脉舒缩张力的作用，探讨其作用机制。方法采用Wistar大鼠离体腹主动脉环（长4～5mm的血管）灌流技术，观察山莨菪碱累积浓度（每5min加入山莨菪碱，使营养液中山莨菪碱浓度分别达3×10^-6，10^-5，3×10^-5，10^-4，3×10^-4mol／L）对单剂量苯肾上腺素（PE）（10^-6mol／L）预处理的血管舒张作用的影响，及不同浓度山莨菪碱（10^-6，10^-5，10^-4mol／L）预孵对累积浓度PE（每5min加入PE，使营养液中PE浓度分别达10^-8，3×10^-8，10^-7，3×10^-7，10^-6，3×10^-6mol／L）收缩血管作用的影响，分别运用一氧化氮合酶抑制剂左旋硝基精氨酸甲酯（L-NAME）（100μmol／L）（对照组加入等容积的蒸馏水）和KATP通道阻断剂格列苯脲（Gly）（100μmol／L）（两对照组分别加入等容积的蒸馏水及溶剂二甲基亚砜DMSO）处理血管环，观察对山莨菪碱舒张血管的抑制作用。结果山莨菪碱累积浓度对单剂量PE预收缩内皮完整及去内皮血管环均有舒张作用，最大舒张分别为（78．6±6．9）％和（65．76±11．39）％，程度相似，差异无统计学意义（P〉0．05）。不同浓度山莨菪碱预孵对累积浓度PE收缩血管环作用呈浓度依赖性抑制，浓度越大，抑制作用越明显。用阻断剂L-NAME及Gly处理后，L-NAME可阻断山莨菪碱的舒张血管（内皮完整）作用（P〈0．05），而Gly对去内皮血管具阻断作用，但只在2min时与对照组差异有统计学意义（P〈0．05）。结论山莨菪碱具有内皮及平滑肌依赖性舒张血管作用，即通过内皮上一氧化氮-环鸟苷酸（NO—cGMP）及平滑肌上α受体发挥作用。山莨菪碱抑制血管收缩主要通过ATP依赖性K^＋通道发挥作用。
Objective To study the effect of anisodamine on contraction and relaxation of isolated abdominal aortic rings in rats and the underlying mechanism. Methods By using the technique of irrigating the Wistar rat＇s abdominal aortic rings, the effect of the accumulated concentration of anisodamine （added anisodamine 3 × 10^-6 ,10^-5 ,3 × 10^-5 ,10^-4 ,3 × 10^-4 mol/L per 5 min） on the 10^-6mol/L phenylephfine （PE） precontracted aortic ring and that of 10^-6, 10^-5 , 10^-4mol/L anisodamine prehatched on precontraeted aortic ring by the accumulated concentration of PE （ added PE 10^-8 ,3 × 10^-8, 10^-7 ,3 × 10^-7, 10^-6 ,3 × 10^-6 mol/L per 5 min） were observed. To deal the aortic ring with L-NAME（ 100 μmol/L） （ added the in the same volume distilled water in the contrasted group） and Gly（ 100 μmol/L） （ added the in the same volume distilled water and DMSO in the two contrasted groups） and observed the inhibition affection on the relaxed blood vessel by anisodamine. Results The accumulated concentration of anisodamine had the relaxed affection on precontracted aortic ring with or without endothelium by single dose PE（ P 〉 0.05 ）. The largest relaxation is （78.6 ± 6.9 ） % and （ 65.76 ± 11.39 ） %. Different concentration anisodamine prehatched was concentration-dependented suppress on the accumulated concentration of PE contracted aortic ring. L-NAME had the affection of blocking the relaxed affection of anisodamine on the aortic ring with endothelium （ P 〈 0.05 ） while Gly had that affection on the aortic ring without endothelium on the 2 minute having the difference compared to the contrasted group （ P 〈 0.0 5 ） . Conclusion Anisodamine had the relaxed affection on blood vessel which dependented endothelium and smooth muscle of the vessel and made the effect on vessel by the NO-cGMP in endothelium and the α-recepter in smooth muscle. Anisodamine surppressed the contracted aortic ring through the ATP-dependented potassium channel.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第4期443-445,共3页
Chinese Journal of Experimental Surgery
基金
福建省自然科学基金资助项目（C0510032）
关键词
山莨菪碱
血管
收缩
舒张
主动脉环
Anisodamine
Vascular
Contraction
Relaxation
Aortic rings
