摘要
利用长瓜把的黄瓜自交系129与短瓜把的Z3杂交衍生的F2株系,检测与瓜把长度相关的QTL。DNA从F2株系提取,并且利用SSR引物对其进行扩增。总共有5个呈现清晰、可重复的多态性分子标记用于QTL检测。结合瓜把数据,利用Mapmaker3.0b构建基因连锁图谱,运用Windows QTL Cartographer V2.0检测QTL。LOD大于2作为QTL存在的阈值。4个来自于同一个连锁群的分子标记CSWGATT01B、CSWGATT01C、CSCT335和CSWGATT01A与瓜把长度显著相关。Qchl1与分子标记CSWGATT01B、CSWGATT01C紧密连锁,与二者分别相距3.4cM和18.0cM,且变异贡献率为18.49%。
A F2 population derived from 129, a cucumber inbred line with long carpopodium, and Z3, a eultivar with short carpopodium, was used to identify quantitative trait loci （QTL） associated with cucumber carpopodium length. DNA was extracted from F2 plants and amplified using simple sequence repeat （SSR） marker. Five polymorphic SSR markers were used for QTL detection and construct the genetic linkage map using Mapmaker 3.0b. QTLs were detected with Windows QTL Cartographer V2.0. Four markers CSWGATT01 B, CSWGATY01 C, CSCT335 and CSWGATT01A that came from one linkage group significantly associated with carpopodium length. Qchll was linked with SSR markers CSWGATT01B and CSWGATT01C by 3.4 cM and 18.0 cM, respectively. It explained 18.49% of phenotypic variation.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第4期543-546,共4页
Acta Horticulturae Sinica
基金
黑龙江省科技攻关项目(GA06B103-8)
黑龙江省博士后基金项目(LBH-Z05032)
关键词
黄瓜
瓜把长度
SSR
QTL
Cucumis sativus
carpopodium length
SSR
QTL
