摘要
目的分析HIV-1+2蛋白印迹试验中HIV-1确证阳性同时出现HIV-2带型样本的基因亚型。方法将HIV-1+2确证试验中同时出现HIV-2带型的样本,通过基因诊断确认是否为HIV-2共感染;同时扩增HIV-1 gag,env基因段,通过测序和系统进化树的构建,分析具有HIV-2带型的HIV-1毒株的基因亚型。结果在34份出现HIV-2带型的标本中,基因诊断发现均为交叉反应,并非HIV-1和HIV-2共感染。出现交叉反应的毒株中以AE亚型为主,占73.5%(25/34),BC重组和B亚型分别占17.7%(6/34)和8.8%(3/34)。结论HIV-1+2蛋白确证试验中HIV-1确证阳性,同时出现HIV-2带型的阳性样本,并非HIV-1和HIV-2共感染,而是交叉反应。出现交叉反应的HIV-1毒株以AE亚型为主。
Objective To study the gene subtype of strain in the cross reaction of HIV - 1 and HIV - 2. Methods Col- lected the HIV - 1 ＋ 2 confirmatory test samples with HIV - 2 band, to confirm if HIV - 2 infection through the gene diagnostic. Amplificated HIV - 1 env and gag gene by PCR;Studied the HIV - 1 gene subtype with HIV -2 cross reaction by Sequencing and polygenetic analysis. Results These samples with HIV - 1 and HIV - 2 band were not HIV - 1 and HIV - 2 co - infection but cross reaction with HIV - 2, Studied the env and gag gene of HIV - 1 with HIV - 2 cross reaction found that AE subtype was 73. 5% （25/34） ; BC subtype was 17.7 % （6/34） ; B subtype was 8.8% （3/34） ; had no other subtype was found in these samples. Conclusion Most HIV - 1 ＋ 2 confirmatory test samples with HIV - 2 bands were not HIV - 1 and HIV - 2 co - infection, but HIV - 1 and HIV - 2 cross reaction. The AE subtype of HIV - 1 was mainly with cross reaction in this research.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2008年第4期198-200,共3页
The Chinese Journal of Dermatovenereology
