摘要
目的：探索兔自体脂肪干细胞、成骨诱导后的脂肪干细胞联合骨形态发生蛋白-2/纤维蛋白胶（bonemorphogeneticprotein-2/Fibringlue,BMP-2/FG）经皮移植到骨缺损后体内成骨能力的差异。方法：实验于2006-08/2007-11在北京军区总医院全军骨科研究所完成。①实验材料：清洁级日本大耳白兔,2.0～2.5kg,雌雄不限,由北京维通利华实验动物技术有限公司提供,实验过程中对动物处置符合动物伦理学标准。②实验方法：制作新西兰大白兔桡骨中段15mm骨缺损模型35侧,随机分为5组,每组7侧：未诱导细胞组、诱导细胞组、未诱导细胞＋BMP-2/FG组、诱导细胞＋BMP-2/FG组、空白对照组。未诱导细胞组、诱导细胞组直接用生理盐水重悬未诱导脂肪干细胞和经成骨诱导的脂肪干细胞,未诱导细胞＋BMP-2/FG、诱导细胞＋BMP-2/FG组分别用含20g/L骨形态发生蛋白-2、5g/L纤维蛋白原的生理盐水重悬未诱导和经成骨诱导的脂肪干细胞。③实验评估：X射线片观察各组骨缺损修复情况,并进行生物力学检测。结果：①12周时,未诱导细胞＋BMP-2/FG组、诱导细胞＋BMP-2/FG组在骨缺损区新生骨的数量明显优于未诱导细胞组、诱导细胞组（P均〈0.05）。②术后4周X射线显示,未诱导细胞＋BMP-2/FG组、诱导细胞＋BMP-2/FG组阻射密度值高于其他组（P均〈0.05）,未诱导细胞组与诱导细胞组无差别。术后8,12周时,诱导细胞组高于未诱导细胞组,未诱导细胞＋BMP-2/FG、诱导细胞＋BMP-2/FG组均高于其他组,诱导细胞＋BMP-2/FG组高于未诱导细胞＋BMP-2/FG组（P均〈0.05）。③生物力学检测显示,未诱导细胞＋BMP-2/FG组、诱导细胞＋BMP-2/FG组桡骨标本的四点弯曲断裂载荷均明显高于其他组,并且诱导细胞＋BMP-2/FG组高于未诱导细胞＋BMP-2/FG组（P均〈0.05）。未诱导细胞组与诱导细胞组无差别。结论：成骨诱导脂肪干细胞及联合BMP-2/FG移
AIM： To explore in vivo osteogenic potential in size-critical bone defect after percutaneous autologous transplantation of culture-expanded rabbit autologous adipose derived stem cells （ADSCs） combined with or without those induced by osteogenic supplement （OS） and with or without bone morphogenetic protein-2/Fibrin glue （BMP-2/FG）. METHODS： Experiments were performed at the Institute of Department of Orthopaedics, General Hospital of Beijing Military Area Command of Chinese PLA from August 2006 to November 2007. （1）Iapanese big ear rabbits （2.0-2.5 kg）, irrespective of gender, were provided by Vital River Lab Animal Technology Co., Ltd. （Beijing, China）. Animal intervention met the animal ethical standards. （2）The 15 mm bone defect experimental models at both radius of New Zealand rabbits were made （35 sides）. Rabbit models were divided into 5 groups at random with 7 experimental models per group： Group A： ADSCs, Group B： ADSCs induced by OS, Group C： ADSCs non-induced by OS＋BMP-2/FG, Group D： ADSCs induced by OS ＋ BMP-2/FG, Group E： blank control. Rabbit models in the Group A and Group B were treated with saline. Rabbit models in the Group C and Group D received saline containing 20 g/L BMP-2/5 g/L FG. （3）Osteogenesis at the defect area was observed by regular radiography and biomechanics examination was performed. RESULTS： （1）A large quantity of callus was found in the Group D and Group C while the others show little callus after cell transPlantation at the 12^th week （P 〈0.05）. （2）The value of X-ray analysis： 4 weeks after operation, the value of Group C and Group D was increased as compared with group A and Group B （P 〈 0.05）. The value of Group A had no difference, as compared with that of Group B. Eight and twelve weeks after operation, the value of Group B was higher than Group A, while the value of Group C and Group D was increased as compared with Group A and Group B respectively. The value of Group D was higher than Group C
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第12期2245-2249,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
