摘要
目的了解携带3种β内酰胺酶基因的鲍氏不动杆菌质粒的可传递性。方法选取从烧伤创面分离出的多重耐药鲍氏不动杆菌（供体菌），将之与大肠埃希菌ATCC25922（受体菌）进行耐药质粒接合、药物敏感试验，并采用PCR分析接合子及其子代的耐药基因型、传代稳定性。结果鲍氏不动杆菌通过接合将其携带对磺胺甲恶唑、氨苄西林、头孢噻吩、头孢博肟、头孢呋辛、亚胺培南／西司他丁和氨苄西林／舒巴坦的耐药性质粒及3种耐药基因传递给受体菌（例如经接合，使受体菌对磺胺甲恶唑的最低抑菌浓度〉2mg／L），且可稳定传代。结论鲍氏不动杆菌质粒上携带可接合传递并稳定传代的β内酰胺酶基因（blaTEM-1、blaPER-1、blaOXA-23），是烧伤感染后其具有多重耐药性的分子生物学机制之一。
Objective To study the transferrable character of Acinetobacter baumanni i（AB） plasraids with 3 types of beta-lactamase gene. Methods The plasmid of multi-drug resistant AB（donor） isolated from burn wound were transferred to E. coil ATCC25922（receptor） through conjugation, and drug sensitivity was also observed. Drug-resistant gene and stability of filial generation and zygote were analyzed by PCR. Results The dug-resistance of donor plasmids to Sulfamethoxazole, Ampicillin, Cefalotin, Cefpodoxime, Cefuroxime, Imipenem/Cilastatin and Ampicillin/Sulbactam,and three types of beta-lactamase gene were transferred to the receptor,and were also stably transmitted for passages. The minimum inhibitor concentration of receptor to Sulfamethoxazole was 〉 2 mg/L after conjugation with donor, and inhibitory character could be transferred to next generation. Conclusion blaTEM-1 , blaPER-1 and blaOXA-23 genes carried in the plasmid of AB can be transferred through conjugation and stably transmitted for passages,and it is one of the molecular mechanisms for AB with multi-drug resistance after burn infections.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2008年第2期97-98,共2页
Chinese Journal of Burns
基金
江西省教育厅科学技术研究项目（200693）
