摘要
应用SSR标记技术对鄱阳湖地区的3种不同斑纹泥鳅的遗传差异进行分析。利用6对特异性引物,在3种斑纹泥鳅中共获得67个微卫星位点,其中在大花斑泥鳅中多态性位点为45个,多态性比例为67.16%,小花斑泥鳅的多态性位点为39个,多态性比例为58.21%,无花斑泥鳅多态性位点为15,多态性比例为22.39%。大、小、无3种斑纹泥鳅的平均期望杂合度分别为0.155 4、0.179 9、0.085 7;平均多态信息含量分别0.273 7、0.362 6、0.198 9。分析显示3种花斑泥鳅间的遗传多样性很低,其中无花斑泥鳅尤甚。从Nei的遗传距离得到的系统聚类树状图显示小花斑泥鳅和无小花斑泥鳅先聚在一起,然后再和大花斑泥鳅聚在一起,结果显示小花斑泥鳅和无花斑泥鳅的亲缘关系更近。分析结果对我们正在开展的泥鳅良种选育具有重要的参考价值。
SSR technique was applied to study the genetic variation among three different of kind spot loaches （ Misgurnus anguillicaudatus ）which were distributed in Poyang Lake of Jinagxi Province. 67 microsatellites DNA loci were detected from the three loaches by using 6 primers. 45 polymorphic loci exist in the big - spot loaches and its percentage is 67.16%. The polymorphic loci of the small - spot loaches is 39 and the percentage is 58.6%. The polymorphic loci of non - spot loaches is 15 and its percentage is 22.39%. The excepted heterozygosity of the big - spot,small - spot and non - spot loaches are 0. 155 4,0. 179 9,0. 085 7, and the PIC are 0. 273 7,0. 362 6, 0. 198 9. Results show that the genetic polymorphic among the three different kind of spot loach are very low, especially in the non - spot loach. The phylogenetic tree was constructed and analyzed by distance method. Results also show that the non - spot loach is more closely related to the small - spot one than to that of the big - spot. It is very important for our researching on the well - bred loach selection from the results all above.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2008年第1期84-88,共5页
Journal of Nanchang University(Natural Science)
基金
国家重点基础研究发展计划资助项目(2004CB117401)
关键词
泥鳅
遗传多态性
SSR
鄱阳湖
loach Imisgurnus angliclli （audatus）
SSR
genetic diversity
the Poyang Lake
