摘要
以樟子松不定芽为外植体,分别对影响樟子松组织培养不定根形成的几个主要因素进行了研究.结果表明:培养基对樟子松不定根的诱导起关键作用,1/4MS培养基有利于不定根的诱导;前期经生长素刺激可诱导不定芽生根,NAA和IBA结合较单独使用具有更佳的生根效果;进一步降低培养基中蔗糖质量浓度,可使生根率提高到72.00%.试验首次在离体培养条件下获得了再生小植株.
This paper made a study of the factors affecting the induction of P. sylvestris var. mongolica roots from adventitious buds. Results show that the eulture medium （1/4 MS being the best） plays a determined role in initiating adventitious roots; that in the early period, auxin can be used to stimulate adventitious buds to take root but the combination of NAA（0.5 mg· L^-1） and IBA （0.2 mg·L^-1） has better effects on rooting ; and that when the concentration of sucrose is reduced to 1%, the rooting rate can reach 72.00%. Accordingly, an experiment successfully induced the first sapling of P. sylvestris vat. mongolica from its adventitious buds.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2008年第1期93-97,共5页
Journal of Central South University of Forestry & Technology
基金
国家林业局项目“干旱地区主要造林树种快速育苗及设备研究”(96-122)
安徽科技学院引进人才基金(ZRC200685)
关键词
生物技术
樟子松
组织培养
不定根
bio-technology
P. sylvestris vat. mongolica
tissue culture
adventitious roots
