摘要
为了找出能够克服或降低高山杜鹃试管苗玻璃化的最适培养条件，本文采用单因子实验设计，以高山杜鹃茎尖作为外植体，Read为基本培养基，研究了培养基中添加物6-BA、蔗糖、琼脂、活性炭的浓度及培养温度等因子对高山杜鹃试管苗生长的影响。结果表明，6-BA、蔗糖、琼脂、活性炭的浓度及培养温度均对试管苗玻璃化率有显著影响。附加了1．5～2．0mg／L6-BA，3％～4％蔗糖，0．6％琼脂，0．3％活性炭的Read培养基和25℃的培养温度能有效得降低高山杜鹃试管苗玻璃化率，而且增殖系数较高、有效苗生长较快。
In order to find out the most suitable culture condition which can control or reduce the vitrification of Rhododendron delavayi in tissue culture, by the design of single factor experiment, the stem apexes of R. delavayi were cultured in Read medium, and several factors effecting vitrification of tissue culture of R. delavayi were studied. The result showed that concentrations of 6-BA, sucrose, agar and AC in the culture medium and culture temperature had a significant influence on vitrification of tissue culture of R. delavayi. The Read medium with 1.5 to 2. 0 mg/L 6-BA, 3% to 4% sucrose, 0. 6% agar, 0.3% AC and the temperature under 25℃ could effectively decrease the rate of vitrification, the coefficient of proliferation was higher and the growth of plantlet quicker.
出处
《西北林学院学报》
CSCD
北大核心
2008年第1期104-107,共4页
Journal of Northwest Forestry University
基金
科技部支撑项目(2005DKA1003)
关键词
高山杜鹃
组织培养
玻璃化
影响因素
Rhododendron delavayi
tissue culture
vitrification
influence factor
