摘要
目的研究间皮素（MESO）在卵巢癌中的表达及意义。方法用逆转录聚合酶链反应（RT—PCR）技术和免疫组化方法分别检测卵巢肿瘤和正常卵巢组织中MESO mRNA及其蛋白水平。结果MESO mRNA和蛋白在上皮性卵巢癌（1．4005±0．4646，2．7857±2．2712）和交界性卵巢肿瘤（1．0650±0．3100，2．9167±2．391）中的表达水平高于良性卵巢肿瘤（0．6463±0．2419，1．2500±1．6125）和正常卵巢组织（0．6439±0．2729，0．9167±1．2401），差异有统计学意义（P〈0．05）；MESOmRNA和蛋白在浆液性卵巢癌（1．5255±0．4151，3．3036±2．6141）和子宫内膜样癌（1．5250±0．5419，3．0000±2．3094）中的表达水平高于黏液样癌（1．0675±0．3149，1．0556±1．9242），差异有统计学意义（P〈0．05）；临床Ⅲ、Ⅳ期MESO表达水平（1．5100±0．4142，3．6087±3．3959）高于I、Ⅱ期（1．1190±0．4909，1．7895±2．6320），差异有统计学意义（P〈0．05）。MESO表达水平与病理分级有关（P〈0．05），而与患者年龄和血清CA125水平无关（P〉0．05）。结论MESOmRNA及蛋白在卵巢癌和交界性肿瘤组表达增高，MESO可能参与卵巢癌的黏附转移。
Objective To investigate the expression of mesothelin （MESO） mRNA and protein and its significance in ovarian carcinomas. Methods Semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry were used to detect the expression level of MESO mRNA and protein, respectively, in 124 samples of ovarian tumor and normal tissues, including 84 epithelial ovarian carcinomas, 1 2 borderline ovarian tumors , 1 6 benign ovarian tumors and 1 2 normal ovarian tissues. Results The expression of MESO mRNA and protein in epithelial ovarian carcinomas （ 1. 4005 ± 0. 4646,2. 7857 ± 2. 2712） and borderline ovarian tumors （ 1. 0650±0.3100, 2. 9167±2.391 ） were significantly higher than that in benign ovarian tumors （0.6463±0. 2419, 1. 2500 ± 1. 6125 ） and normal ovarian tissues （0.6439 ± 0.2729, 0. 9167 ± 1. 2401 ） （ P 〈 0. 05 ）, and also significantly higher in serous cystadenocarcinoma （ 1. 5255 ± 0.4151,3. 3036 ± 2.6141 ） and endometrioid carcinoma （ 1. 5250 ± 0.5419, 3. 0000 ± 2. 3094 ） than that in mueinous eystadenoeareinoma （ 1. 0675 ± 0.3149,1. 0556 ± 1. 9242 ） （ P 〈 0.05 ）. The expression of MESO mRNA and protein in stages Ⅲ and Ⅳ carcinomas （1. 5100±0.4142,3. 6087 ± 3. 3959） was significantly higher than that in stages I and II carcinomas （ 1.1190 ± 0.4909,1. 7895 ± 2.6320 ;P 〈 0.05） , and also significantly higher in grade 3 carcinomas than that in grade 1 and 2 ones （P 〈0.05 ） , but was not correlate with age or serum CA125 of the patients （P 〉 0.05 ）. Condusion The results of this study demonstrated that the expression of MESO mRNA and protein is increased in ovarian carcinomas and borderline ovarian tumors, and MESO may play a role in the adhesion and dissemination of ovarian carcinomas.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2008年第4期288-291,共4页
Chinese Journal of Oncology
