摘要
目的探讨利用链脲佐菌素(STZ)建立理想糖尿病性勃起功能障碍大鼠模型。方法30只SD大鼠,随机分为5组,分别为对照组、注射STZ40mg/kg组、60mg/kg组、80mg/kg组、100mg/kg组,每组6只,分别记录4d、1周、2周、3周的空腹血糖、阿朴吗啡诱导阴茎的勃起次数及体质量。结果4个时间点间的空腹血糖有显著性差异(P=0.001),不同STZ注射组别间的空腹血糖、勃起次数及体质量改变存在显著性差异(P<0.001,P=0.045及P<0.001),阿朴吗啡阴茎勃起实验的勃起次数及体质量的改变在4个时间点间不存在显著性差异(P=0.306和P=0.628)。结论最佳成糖尿病模型的STZ注射剂量应为60mg/kg,筛选糖尿病性勃起功能障碍模型的阿朴吗啡勃起实验筛选时间应定在注射STZ后的2周左右。
Objeetive To establish a rat model of diabetic erectile dysfunction （ED） with streptozotocin （STZ） injection. Methods Thirty male rats were randomized equally into 5 groups （control group and STZ 40 mg/kg, 60 mg/kg, 80 mg/kg, and 100 mg/kg groups）. All the rats were examinaed at 4 days and 1, 2, and 3 weeks after STZ injection for fasting blood glucose, erectile frequency induced by apomorpHine （APO） and body weight changes. Results Significant difference occurred in the fasting food glucose among the groups at different time points （P=0.001）, and also in APO-induced erectile frequency, fasting blood glucose and body weight between the groups with STZ injection at different doses （P〈0.001, P=0.045 and P〈0.001, respectively）. No significant difference was found in induced erectile frequency and body weight between different time points （P=0.306 and P=0.628）. Conclusion The optimal dose of STZ for establishing diabetic ED model is 60 mg/kg, and two weeks after the injection can be the optimal time for evaluating model establishment by means of APO-induced penis erection.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第4期564-566,共3页
Journal of Southern Medical University
基金
全军十五科技计划基金(03M105)
广东省自然科学基金(5B1040144)
