摘要
目的探讨红细胞冻干长期保存的有效方法,并评价复水后红细胞各项理化指标的变化。方法设对照组(常规条件下保存的红细胞)和实验组(负载海藻糖冻干-复水后红细胞),在37℃条件下,红细胞负载海藻糖7h后,采用主要成分为含15%聚乙烯吡咯烷酮(PVP)和150mmol/L海藻糖的缓冲液作为保护液,在设定的降温程序下进行红细胞的冻干保存。冻干后置37℃的再水化液快速水化,检测各项理化指标。结果红细胞冻干再水化后红细胞和血红蛋白回收率均在80%以上,且各项理化指标与常规保存的对照红细胞间差异无显著性(P>0.05)。结论红细胞在37℃孵育7h的条件下负载海藻糖后进行冻干,复水后能保持细胞的理化稳定性和结构形态的完整性,为进一步研究长期冻干保存红细胞奠定了基础。
Objective To explore an efficient technology for long-term preservation of red blood cells and evaluate the biological activities of the RBCs after lyophilization. Methods The samples were divided into two groups：the control group （RBCs preserved in routine method）, and the test group （red blood cells after freeze-drying and rehydration）. Loading RBCs at 37℃ in a time span of 7 h,then put the RBCs into the protective solution which mainly composed of 150 mmol/ L trehalose, 15% polyvinylpyrrolidone （PVP） and isotonic buffer were adopted to lyophilize red blood cells at designed pro- cedure. After lyophilization,the samples were quickly rehydrated by 37℃ rehydration solution. RBCs recovery and the activity level of ATP and G-6-PD were investigated. Results The results indicated that the survival of RBCs after lyophiliza-
出处
《临床输血与检验》
CAS
2008年第2期106-109,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
国家自然科学基金课题(No30471931)资助
