摘要
通过免疫组织化学方法,应用CD3、CD4和CD8单克隆抗体研究了CD3+T淋巴细胞及CD4+和CD8+T淋巴细胞亚群在鸡盲肠扁桃体中的出现、迁移、组织定位分布及数量变化规律等一系列发育过程。结果显示,CD3+、CD8+T淋巴细胞最初于20日龄胚胎中出现,而CD4+T淋巴细胞在出壳后1日龄时出现。在定位分布变化上,4日龄之前,T淋巴细胞主要分布在黏膜层的中下部区域,而4日龄以后的各日龄在整个黏膜层中均匀分布,CD4+细胞主要聚集在滤泡间区域,而CD3+细胞和CD8+细胞在固有层中和黏膜上皮中都有大量分布。在数量变化上,4日龄时,各种阳性细胞的数量都骤然增加,而到7日龄时,CD3+、CD8+T淋巴细胞的数量明显减少,CD4+细胞的数量无明显变化,从14日龄开始直到35日龄,CD3+、CD4+和CD8+T淋巴细胞的数量持续增加。证实,鸡出壳后初期盲肠扁桃体的细胞免疫功能增强,并在14日龄时达到成熟水平。
Occurrence, migration, tissue localization and quantity changes of CD3^＋ ,CD4^＋ ,and CD8^＋ T lymphocyte subpopulations in chicken caecal tonsil was examined immunohistochemically by using the CD3,CD4 and CD8 monoclonal antibodies. The findings showed that both CD3^＋ and CD8^＋ T lymphocytes appeared firstly in caecal tonsil of embryo of 20-day-old and CD4^＋ cells appeared in 1-day-old broiler. T lymphocytes were distributed mainly in the middle and underneath of mucous layer before 4-day-old, but they distributed uniformly in the whole mucous layer after 4-day-old,CD4^＋ cells concentrated in the interfollicular areas,and both CD3^＋ and CD8^＋T lymphocytes distributed in the lamina propria and epithelium. Various kinds of positive cells increased rapidly in 4-day-old broiler, but they significantly decreased in 7- day-old broiler. Although the number of CD4^＋ cells had no marked change, their number kept increasing from 14-day-old to 35-day-old. The results revealed that the cellular immune function of caecal tonsil in chicken strengthened fast after hatching,and reached the mature level in 14 day-old broiler.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第4期338-341,共4页
Chinese Veterinary Science
基金
国家自然科学基金项目(30771557)
关键词
鸡
盲肠扁桃体
T淋巴细胞
亚群
发育
chicken
caecal tonsil
T lymphocyte
subpopulation
development
