摘要
利用生物信息学和比较基因组学方法对鸡pATGL(predicted adipose triglyceride lipase)基因的结构、表达和功能进行了分析,结果表明:(1)电子克隆获得的鸡pATGL基因cDNA全长1 788 bp,包括1 452 bp的编码区和5′UTR(70 bp)、3′UTR(266 bp),编码合成483个氨基酸的多肽;(2)鸡pATGL基因全长在30 kb以上,包含9个外显子和8个内含子,其中内含子1(约20 kb)远远长于其他区域;(3)鸡pATGL蛋白的相对分子质量为53 600,不含信号肽,具有GXSXG(氨基乙酸-X-丝氨酸-X-氨基乙酸)脂肪酶活性结构和Patatin结构域,可能存在5个N端朝内的跨膜螺旋结构;(4)基于cDNA及EST数据库的检索显示,鸡pATGL基因在腹脂、胸腺、黏液囊、脾、骨髓、垂体、下丘脑和松果腺、心脏、软骨组织、小脑、卵巢乃至胚胎等组织中表达;(5)鸡pATGL基因与CD151、RPLP2位于5号染色体的紧密连锁群,这种连锁关系与人、小鼠以及狗紧密一致.
Using bioinformatic and comparative genomic methods, the structure, expression and function of the chicken pATGL （predicted adipose triglyceride lipase） gene was analyzed in this study. Results showed that： （1） The obtained pATGL gene eDNA by electronic cloning was 1 788 bp long and contained 70 bp of 5＇UTR, 266 bp of 3＇UTR as well as 1 452 bp of open reading frame that encoded a 483 amino acid peptide. （2） The chicken pATGL gene was 30 kb in full length and comprised 9 exons and 8 introns, in which intron 1 （20 kb） was far longer than others. （3） Bioinforrnatic analysis showed that chicken pATGL, with the relative molecular mass of 53 600, had a ＂GXSXG＂ （ Glycine-X-Serine-X-Glycine） lipase-containing box, a patatin-like region and five possible transmenmber helix structure, howev-er no signal peptide was found. （4） Based on cDNA and EST library, the chicken pATGL gene was expressed in abdominal fat pad, thymus, bursa, spleen, PBL, bone marrow, embryo, head, pituitary gland, hypothalamus, pineal gland, heart, growth plate cartilage, brain and ovary. （5） The close linkage of pATGL gene with CD151 and RPLP2 was observed in chicken, which was conserved in other species, such as human, mouse and dog.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2008年第2期94-98,共5页
Journal of South China Agricultural University
基金
"863"计划项目(2006AA10A120)
国家自然科学基金(30600429)
