摘要
逆流色谱是一种无固态支撑物的液液色谱 ,它的色谱动力学过程有其特殊性 ,分析逆流色谱的动力学过程 ,推导适用于逆流色谱的速率方程式 ,结合实验 ,讨论影响色谱峰展宽的因素 ;理论推导和实验的结果都显示 ,管径、容量因子。
Dynamics in countercurrent chromatography, a kind of liquid liquid chromatography without solid matrix, is very different to that in other liquid liquid chromatography. Dynamics in countercurrent chromatography was analyzed and the rate equation that fits countercurrent chromatography was derived out. Some experiments were done to measure H(plate height) of two kinds of CCPC(centrifuge countercurrent partition chromatography) columns. The results of both theoretical deriving and experiments reveal that the diameter of column, capacity factor, mass transfer coefficient, the average diameter and velocity of mobile phase droplets are main factors that determines H. Mathematics analysis is given.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1997年第2期144-147,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目
关键词
逆流色谱
动力学
分离效率
countercurrent chromatography, dynamics, centrifuge
