摘要
应用出血热病毒的抗核蛋白（ＮＰ）和糖蛋白（Ｇ１、Ｇ２）单克隆抗体的ＥＬＩＳＡ夹心法对感染细胞培养物和灭活疫苗内的ＮＰ和Ｇ１、Ｇ２抗原成分进行检测，结果表明感染Ｖｅｒｏ－Ｅ６细胞内ＮＰ和Ｇ１、Ｇ２含量均高于细胞外培养液上清，前者的抗原滴度分别为≥５１２和２５６，后者仅为６４和１６。比较三种不同疫苗内的抗原成分，显示乳鼠脑纯化疫苗内的ＮＰ抗原最高，滴度达３２０－≥６４０，而二种细胞疫苗（沙鼠肾和地鼠肾细胞）则较低，一般在２０－８０，相反二种细胞疫苗的糖蛋白滴度则高于脑疫苗（８－３２对２－８）该方法可用于疫苗生产过程中检测ＮＰ和Ｇ抗原成分。
A method of sandwish ELISA using anti-NP and G 1,G 2 monoclonal antibodies was established for detection of antigen components in HFRS virus infected cell culture and vaccines.The results revealed that higher titers of NP and G(G 1+G 2)components can be detected in the infected Vero-E 6 cells but lower titer in the supernatant of cell culture(≥512 & 256 vs 64 & 16).Higher NP component was detected from purified mouse brain (PMB)vaccine than from both tissue culture vaccines (primary Gerbil kidney and Hamster kidney cells) (320-≥640 vs 20-80).In contrast higher G titer was detected from the both cell vaccines than from the PMB vaccine (8-32 vs 2-8) This method can be used in process of vaccine production for detection NP and G components in vaccine.
出处
《微生物学免疫学进展》
1997年第3期43-45,共3页
Progress In Microbiology and Immunology
