摘要
以青岛百合叶片为外植体,研究了消毒时间、培养基、生长调节物质对青岛百合叶片不定芽发生、增殖和生根的影响。结果表明:用0.1%氯化汞对青岛百合叶片消毒5min时效果最好,污染率为31%,萌动率为79%,诱导率为65.2%。青岛百合最佳叶片不定芽分化培养基为MS+6-BA2.0mg/L+NAA0.2mg/L;最佳不定芽增殖培养基为MS+6-BA1.0mg/L+NAA0.2mg/L;最佳生根的培养基为1/2MS+NAA0.2mg/L,形成了较为完善的组织培养再生体系。
The leaf of Lilium tsingtauense Gilg. were used as experimental materials. And the effect of different sterilized time, kinds of hormone and its combination on advenitious buds proliferation, plant regeneration and root regeneration were studied the in this paper. The results showed that explant sterilized in 0.1% aqueous mercuric chloride for 5 min had better effect, and the contamination rate was 31%, the germinating rate was 79%, inducement rate was 65.2%. MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L was suitable for advenitious buds proliferation from the leaf. The optimum medium was MS＋6-BA 1.0mg/L＋NAA 0.2mg/L. The best medium for rooting was 1/2MS＋NAA 0.2mg/L. The comparatively perfect tissue culture regeneration system was formed.
出处
《中国农学通报》
CSCD
2008年第4期85-88,共4页
Chinese Agricultural Science Bulletin
基金
山东省农业良种工程重大项目(鲁科农字[2005]99号)
关键词
青岛百合
叶片
组织培养
增殖
生根
Lilium tsingtauense Gilg., leaf, tissue culture, proliferation, rooting
