摘要
目的探讨脑出血灶周缺氧诱导因子-1a(hypoxia inducible factor-1a,HIF-1a)和血清促红细胞生成素(erythropoietin,EPO)的表达及意义。方法采用免疫组化法、放射免疫法分别检测31例脑出血灶周脑组织中HIF-1a及血清EPO的表达,并与健康对照组17例比较。结果31例脑出血灶周HIF-1a表达率为41.94%,与对照组比较差异有统计学意义(P<0.01);HIF-1a随出血量增加表达随之升高,大、中量组与对照组及大量组与小量组比较差异有统计学意义(P<0.05或P<0.001)。EPO随出血量增加表达随之降低,但均高于对照组,且差异有统计学意义(P<0.05或P<0.001);大、中量组与小量组比较差异有统计学意义(P<0.01)。HIF-1a随手术时间推迟表达升高,而EPO表达和病程无相关性。HIF-1a和EPO呈负相关(r=-0.432,P<0.05)。结论脑出血后HIF-1a、EPO的表达有明显改变,在灶周低氧所致血管新生过程中可能起重要作用。
Objective To explore expression and significance of hypoxia inducible factor-1a （HIF-1a）and serum erythropoietin （EPO） in cerebral hemorrhagcal perifocus. Methods Expression of HIF-1a and serum EPO were assessed by immunohistochemical straining and radioimmunoassay in 31 cases with hypertensive hemorrhage and were compared to the normal group eontaining 17 cases. Results In 31 cases of hypertensive hemorrhage,the expressional rate of HIF-1a was 41.94 %. There was significant difference compared to normal group （ P 〈0.01 ）. Expression of HIF-1a got higher as the volume of hemorrhage increased. There were significant difference between the massive-intermediate volume group and the control group, and between the massive volume group and the small volume group （P 〈 0.05 or P 〈 0. 001 ）. Expression of EPO got lower as the volume of hemorrhage increased, but were all higher than those in the control group. There was significant difference between the massive to intermediate volume group and the small volume group （ P 〈 0.01 ）. Expression of HIF-1a got higher with the delay of operation time. There was no correlation between the expression of HIF-1a and course of illness. There was a negativly correlation between HIF-1 a and EPO （ r = - 0.432, P 〈 0.05 ）. Conclusions Expression of HIF-1a and EPO changed significantly after cerebral hemorrhage. It may have important effect on the course of pefifocus leading to vasoneogenesis.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2008年第1期30-32,共3页
Journal of Apoplexy and Nervous Diseases
