摘要
目的：建立测定肺炎平胶囊中原儿茶酸和原儿茶醛的含量测定方法。方法：采用高效液相色谱法,色谱柱为Diamonsil-C18（4.6 mm×250 mm,5μm）,流动相为甲醇-1%醋酸溶液（11∶89）,流速为1.0 mL·min^-1,检测波长为281 nm,柱温40℃。结果：原儿茶酸和原儿茶醛线性范围分别是：（0.082 4～0.824）μg,r=0.9999;（0.012 4～0.124）μg,r=0.9997。平均回收率分别为97.67%,101.68%;RSD为1.31%,2.04%。结论：该法分离好,快速、简便,可作为该产品的质量控制方法。
Objective： To establish a method for determination of protocatechuic acid and protocatechualdehyde in Feiyanping Capsule. Methods： The HPLC separation was performed on Diamonsil-C18 （4.6 mm×250 mm, 5 μm） column with a mixture methanol - 0.1% acetic acid solution （ 11 ： 89） as the mobile phase, at a flow rate of 1.0 mL·min^-1 .Detection wavelength was at 281 nm, and column temperature at 40 ℃. Results： The linear responses of protocatechuic acid and protocatechualdehyde were at a range of （0.082 4 - 0.824）μg, r = 0.999 9 and （0.012 4 - 0.124） μg, r = 0.999 7; the average recoveres were 97.67%, 101.68%; RSD 1.31% and 2.04% respectively. Conclusion：The method is simple, accurate, and suitable for quality control of Feiyanping Capsule.
出处
《中国实验方剂学杂志》
CAS
2008年第3期8-9,12,共3页
Chinese Journal of Experimental Traditional Medical Formulae
