摘要
目的：探讨高血压性脑出血患者血肿周围脑组织缺氧诱导因子-1α（HIF-1α）在早期脑水肿形成中的作用和机制。方法：血肿清除术治疗的32例高血压性脑出血患者血肿周围脑组织，采用免疫组织化学染色技术测定HIF-1α和血管内皮生长因子（VEGF）的表达；根据术前头颅CT测量血肿周围脑水肿体积；采用双盲法对染色结果和脑水肿体积进行分析。结果：出血4h可见神经元和血管内皮细胞肿胀，血肿周围脑组织可见散在的HIF-1α阳性神经元，出血24～48h时HIF-1α表达达高峰，49～72h持续高表达，与正常脑组织相比存在显著差异（P〈0．01）。HIF-1α表达与VEGF表达（r=0．72，t=6．37，P〈0．01）和脑水肿体积（r=0．64，t=4．56，P〈0．01）呈正相关。结论：高血压脑出血血肿周围脑组织HIF-1α表达与早期脑水肿形成有关，HIF-1α可能通过诱导和调控VEGF表达促进脑水肿形成。
Objective： To investigate the effect and mechanism of hypoxia-inducible factor-1α （HIF-1α） in perihematomal brain tissue on the early formation of edema in patients with hyper- tensive intracerebral hemorrhage. Methods： The perihematomal brain tissue in 32 patients with hypertensive intracerebral hemorrhage were treated with hematoma evacuation. The expressions of HIF- 1α and vascular endothelial growth factor （VEGF） were measured by immunohistochem- istry staining technique. The volume of perihematomal edema was determined on the basis of preoperative head CT scan. The results of staining and the volume of edema were analyzed by using double-blind method. Results： Neuron and vascular endothelial cell swelling were observed 4 hours after cerebral hemorrhage, and the scattered HIF-1α positive neurons were visible. The expressions of HIF-1αreached the peak at 24 to 48 hours, and went on high expressions at 49 to 72 hours. There was significant difference as compared with the normal brain tissue （P 〈 0.01）. There were positive correlations between the expressions of HIF-1α and VEGF （r = 0.72, t = 6. 37, P 〈0.01） and the volume of brain edema （r =0.64, t =4.56, P 〈0.01）. Conclusions： The expression of HIF-1α in perihematomal brain tissue of hypertensive cerebral hemorrhage is associated with the early formation of cerebral edema. HIF-1α may accelerate the formation of brain edema by inducing and regulating the expression of VEGF.
出处
《国际脑血管病杂志》
2008年第3期181-184,共4页
International Journal of Cerebrovascular Diseases
关键词
缺氧诱导因子-1Α
血管内皮生长因子
脑出血
脑水肿
hypoxia-inducible factor-lot
vascular endothelial growth factor
intracerebral hemorrhage
brain edema
