摘要
目的观察Nogo-A受体拮抗剂NEP1-40及神经节苷脂对新生大鼠缺血缺氧性脑损伤后神经再生的作用。方法将100只7日龄新生大鼠随机分为10组:在6 h、24 h两个时间段分别设空白对照组,假手术组,缺氧缺血性脑损伤(HIBD)模型组,HIBD后NEP1-40治疗组及神经节苷脂治疗组,用原位杂交的方法观察各组别Nogo-A mRNA的表达情况。结果HIBD组两时段的Nogo-mRNA表达均高于同时段空白组及假手术组,差异有统计学意义;NEP1-40治疗组能抑制HIBD后mRNA的表达,与同时段模型组对比差异有统计学意义;神经节苷脂治疗组mRNA的表达在6h时与HIBD组无明显差异,在24h时较HIBD组低但高于空白组。P值均<0.05。结论Nogo-A mRNA在缺氧缺血性脑损伤后表达显著增高,其编码的Nogo-A可抑制中枢神经损伤后的再生,NEP1-40能拮抗这一作用,从而可能在促进缺氧缺血性脑损伤后神经再生中起到作用。神经节苷脂GM-1在缺氧缺血性脑损伤后也可抑制Nogo-A mRNA的表达,有稳定细胞膜、减轻细胞水肿、促进神经再生的作用。
Objective To investigate the effects of NEP1-40 and GM-1 in newborn rats with hypoxic ischemic brain damage （HIBD）. Methods 100 rats were randomly divided into 10 groups and five groups were treated in different ways at 6 h： the normal control group, the sham-operation group, the HIBD model group, the GM-1 treatment group and the NFP1-40 treatment group, and the other five groups were treated at 24 h. Contents of Nogo-A mRNA in each group were determined by the method of in situ hybridization. Results The expression of Nogo-A mRNA in the HIBD groups was higher than that in the control groups, and was lower in the NEP1-40 groups than in the HIBD groups at both time points. There were no significant differences between the GM-1 group and the HIBD group when they were treated at 6 h. However, the expression of Nogo-A mRNA was lower in the GM-1 group than in the HIBD group but was higher than the normal control group. Conclusion Nogo-A mRNA is sig- nificantly increased in newbom rats with HIBD. Nogo-A encoded by mRNA can inhibit the regeneration of the central nerve after injury, and NEP1-40 could antagonize the function and promotes the regeneration. GM-1 is also able to antagonize the expression of mRNA and stabilizes the cellular membrane, eases cellular edema of the injured nerve and accelerates the regeneration of new nerves.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第3期240-244,共5页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金(Z2005C03)
2005年山东省医药卫生科技发展计划
