摘要
目的:探讨选择性环氧化酶2(COX-2)抑制剂尼美舒利抑制胃癌细胞株SGC7901增殖的作用和可能的细胞内信号转导机制。方法:将选择性COX-2抑制剂尼美舒利作用于胃癌细胞株SGC7901,MTT法检测细胞增殖状态;流式细胞仪检测细胞周期及凋亡;Western blot法检测STAT3通路相关蛋白的表达。结果:尼美舒利作用胃癌细胞株SGC7901后细胞增殖受到显抑制且呈时间、剂量依赖性方式;细胞凋亡百分数增高(P<0.05);G_0/G_1期细胞比例升高(P<0.05);Western Blot结果显示SGC7901细胞中磷酸化STAT3(P-STAT3)、CyclinD1、Bcl-2蛋白的表达明显降低(P<0.05)。结论:尼美舒利可抑制胃癌细胞株SGC7901的增殖,阻滞细胞周期的进展,促进细胞凋亡.其机制可能与抑制STAT3信号通路相关蛋白P-STAT3、CyclinD1、Bcl-2表达有关。
Objective：To investigate the inhibition effect of proliferation of gastric cancer cell line SGC7901 treated with selective cyclooxgenase 2（COX-2 ） inhibitor, nimesulide, and the probable mechanism of cell signal transduction. Methods： SGC7901 cells were treated with nimesulide. MTT assay was used to measure the cell proliferation;Flow cytometry was used to detect cell cycle and apoptosis. The protein expression related to STAT3 signaling pathway in SGC7901 cells was examined by Western blot. Results：The growth of gastric cancer cells was obviously inhibited in a time-and dose-dependent manner after nimesulide treatment;the percentage of apoptosis was increased significantly（P〈0.05）;the proportion of SGC7901 in G0/G1 phase was hoisted significantly（P〈0.05）;Western blot analysis showed that：the protein expression of phosphorylated STAT3 （P-STAT3）, CyclinD1,Bcl-2 in SGC-7901 cells was decreased significantly（P〈0.05）. Conclusions：The proliferation of gastric cancer cell line SGC7901 could be inhibited;the course of cell cycle could be prevented;cell apoptosis could be induced by nimesulide through down-regulating the protein expression of P-STAT3,CyclinD1, Bcl-2,which was probably one of its molecular mechanisms.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第3期294-296,333,共4页
Journal of Chongqing Medical University
基金
重庆市教委科研基金(2003)
关键词
信号转导和转录激活因子3
胃癌
尼美舒利
Signal transducer and activator of transcription 3
Gastric cancer
Nimesulide
