摘要
目的建立人血浆中西替利嗪浓度的HPLC测定法。方法色谱柱为Hypersil ODSC18（150mm×5．Omm，5μm）；流动相为0．02mol·L^-1。磷酸二氢钾溶液（pH3．50）：乙腈=77：23；流速1．0mL．min^-1；柱温30℃，检测波长230nm。结果西替利嗪血浆浓度在20～1000μg．L^-1。浓度范围内，线性关系良好（r=0．9998）。方法回收率99．54％～102．27％，日内、日间RSD均〈10％。结论本方法简单、灵敏、重现性好，适用于临床血药浓度监测．以及人体药动学的研究。
OBJECTIVE To establish an HPLC method for the determination of cetirizine in the blood plasma. METHODS The analysis was carried out by using Hypersil Cl8 column（5ttm, 150mm × 5.0mm）. The mobile phase consisted of 0.02mol· L^- 1 potassium dihydrogen phosphate（pH 3.50）-acetonitrile = 77 ： 23. The flow rate was 1.0mL·min^-1. The column temperature was 300C. The excitation wavelength was 230nm. RESULTS The linear range of cetirizine was 20 -- 1000μg·L^-1 （r = 0. 9998）, The recoveries were 99.54 % -- 102.27 %, and RSD of intra-and inter-day were below 10 % .CONCLUSION The method is practical and accurate, and can be applicated in clinical blood medicine assay and the pharmacokinetics of human body.
出处
《海峡药学》
2008年第3期119-120,共2页
Strait Pharmaceutical Journal
