摘要
目的:观察维生素C、E对五周龄生长期染铅SD大鼠血铅及海马超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、NOS活性及丙二醛(MDA)、NO含量的影响。方法:大鼠自由饮用0.615mmol/L的醋酸铅4周造成铅中毒模型。然后以维生素C 100 mg/kg、维生素E 100mg/kg单独或联合灌胃1周。测量血铅水平,大鼠海马组织SOD、GSH-Px、NOS活性及MDA和NO含量。结果:与铅模型组比较,给予维生素C,维生素E后,大鼠血铅浓度显著性降低(P<0.05);MDA含量下降,且其联合治疗组与单独维生素C治疗组差异有显著性(P<0.05);SOD,GSH-Px、NO、NOS水平显著高于铅模型组,差异有显著性(P<0.05)。结论:补充维生素C、维生素E可以降低血铅含量,减轻铅中毒引起的海马的脂质过氧化损伤,提高NOS、NO活性。
Objective： To observe the effect of vitamin C and E on blood lead （Pb） levels and SOD,GSH-Px,NOS activity and NO,MDA content in hippoeampus of rats with lead poisoning. Methods： Rat lead poisoning model was established by oral administration of 0. 615 mmol/L lead acetate in drinking water for 4 weeks; and animals were fed with vitamin C 100 mg/kg, bw and/ or vitamin E 100 mg/kg, bw for 1 week. Then blood Pb levels and SOD,GSH-Px,NOS activity and MDA,NO contents in hippocampus of rats were determined by corresponding kits. Results： Compared with control group,blood Pb level was decreased significantly （P〈0. 05） after given vitamin C,vitamin E or combination of vitamin C and E. The concentrations of SOD,GSH-Px,NO and NOS were significantly higher in vitamin C and/or E groups than those in control group （P〈0. 05）. The concentration of MDA in vitamin treatment groups was significantly lower than that in lead control group （P〈0.05） ; furthermore concentration of MDA in combination of vitamin C and E group was significantly higher than that in vitamin C alone group （P〈0. 05）. Conclusion： Administration of vitamin C and E can decrease blood lead level, alleviate damage of lipid peroxidation in hippocampus by lead toxicity and reverse NO, NOS levels in rats with lead poisoning.
出处
《浙江大学学报（医学版）》
CAS
CSCD
2008年第2期189-192,共4页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省卫生厅科研基金资助项目(2005A069)
