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两株死胎源猪圆环病毒Ⅱ型的分离与序列分析 被引量:1

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摘要 将两猪场Ⅱ型猪圆环病毒(PCV2)核酸阳性的流产死胎病料,接种猪肾传代细胞PK15,成功分离到2株PCV2。利用1对PCV2特异性引物,成功扩增出这2株PCV2长约1700bp的全基因组序列,并与其他PCV2毒株序列进行相似性比较。结果表明:2个分离株全基因组长度均为1767bp,核苷酸序列相似性为99.9%;ORF2基因核苷酸相似性为99.85%,所编码的衣壳蛋白氨基酸序列相似性为100%;2个分离株与SD-3等国内分离株核苷酸序列的相似性较高,最高可达99.9%。
出处 《广东畜牧兽医科技》 2008年第2期36-39,共4页 Guangdong Journal of Animal and Veterinary Science
基金 广东省科学技术计划项目(2007A020300006-3)
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