摘要
为了简化烟叶样品前处理步骤,采用正己烷直接萃取烟叶样品得到烟叶的正己烷萃取液,利用KOH水溶液对正己烷萃取液直接进行油水两相皂化,再采用高效液相色谱法测定皂化后萃取液中的茄尼醇浓度。结果表明：①萃取温度65℃,萃取时间24 h,KOH水溶液浓度0.01 mol/L,皂化时间0.5 h时,萃取效率较好,皂化转化率较高;②茄尼醇的质量浓度范围为2.125-34.000 mg/L时,峰面积与质量浓度具有良好的线性关系,相关系数为0.99998;③平均回收率为97.6%,相对标准偏差（RSD）为2.7%（n=3）。表明该方法快速、简便、准确。
In order to simplify the pretreatment of tobacco leaf samples, leaf sample was directly extracted by hexane and the obtained extract was saponified with potassium hydroxide, the solanesol concentration in saponified extract was determined by HPLC. The result showed that： 1） the efficiencies of extraction and saponification were better when the extraction lasted 24 hrs at 65 ℃ and the extract was saponified with 0.01 mol/L KOH for 0.5 hrs; 2） the peak area had good linear relationship with mass concentration in the concentration range of 2. 125 to 34. 000 mg/L （r = 0. 99998） ; 3） the average recovery was 97.6% and the relative standard deviation （RSD） was 2.7% （n =3）. The method was rapid, simple and accurate.
出处
《烟草科技》
EI
CAS
北大核心
2008年第3期49-52,共4页
Tobacco Science & Technology
关键词
茄尼醇
高效液相色谱
烟叶
萃取
皂化
Solanesol
HPLC
Tobacco leaf
Extraction
Saponification
