摘要
目的:探讨如何成功建立小鼠心肌梗死模型,提高动物的存活率。方法:采用结扎冠状动脉左前降支方法建立小鼠心肌梗死模型,术后4周取出心脏对心肌进行TTC和HE染色检测。结果:通过对常规手术方法进行改进,术后2周小鼠存活率由40%提高到75%。心肌颜色、心电图特异性改变及心肌TTC和HE染色证明制模成功。结论:正确使用麻醉药、顺利气管插管、准确结扎冠状动脉、减少出血与肺损伤及术后观察和护理是保证制模成功的基础。
Objective：To investigate how to establish a credible mice model of myocarclial infarction with a high survival rate.Methods：Develope mice model of myocardial infarction by ligation the left anterior descending coronary artery,and the myocardium was detected by TTC and HE staining in 2 weeks after operation.Results：Following the traditional operational method was improved,the survival rate of mice was improved from 40% to 75%.The changes of echocardiography、myocardium colour、TTC and HE staining proved the success of model.Conclusion：The factors,including correct applying of anaesthetic、favorable intubation、exact ligating of left anterior descending coronary artery、decreaseing bleeding and lung injury、observing and nursing after operation,were basis for establishing mice model with myocarclial infarction.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第6期631-633,共3页
Journal of China Medical University
基金
国家"973"项目(2001CB509904)
关键词
心肌梗死
小鼠
模型
myocardial infarction
mouse
model
