摘要
目的:分析子宫内膜异位症(EMs)患者血清差异蛋白谱,初步建立EMs的诊断模型。方法:采用表面增强激光解析离子化飞行时间质谱(SELDI-TOF-MS)技术,用弱阳离子交换芯片(WCX2)和分析软件对79例EMs(EMs组)及72例非EMs妇女(对照组)血清样品进行检测,在不同的月经周期中分别筛选差异蛋白,建立诊断模型并进行盲法验证。结果:在质荷比(m/z)2000-30000的范围内,得到16个有统计学差异(P<0.01)的差异蛋白,进一步按月经周期分期分析,增生期和分泌期分别得到3个和6个有统计学差异的蛋白峰。增生期所建的诊断模型敏感性和特异性分别为94.3%和97.1%。盲筛结果敏感性为90.7%,特异性为93.0%,阳性预测值为92.9%,阴性预测值为90.9%。结论:利用增生期血清样品建立的诊断模型敏感性和特异性高,对EMs临床诊断和发病机理的理解具有重要意义。
Objective： To analyze differential proteins in serum of patients with and without endomvtriosis （EMs） and establish a pattern for diagnosis of endometriosis. Methods： Differential proteins of serum samples between endometriosis group（79 samples） and control group （72 samples） were detected using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS）, weak cation exchanger （WCX2） protein chip and Biomarker Wizard software, then diagnostic models were established in different menstrual cycle. Results： At the m/z value ranging from 2 000 to 30 000, 16 different protein fmgcrprints which were of statistical significance （P〈0.01） were detected without considering the menstrual cycle. Between the two groups in proliferative phase, 3 proteins had significant difference, 6 proteins had significant difference between samples in secretory phase. The sensitivity and specificity of the model of proliferative phase were 94.3 % and 97.1%, respectively. The blind test generated a sensitivity of 90.7%, specificity of 93.0%, positive and negative predictive value were 92.9% and 90.9%, respectively. Conclusion： The diagnostic model established according to the differential protein fingerprints in proliferative phase is the most valuable. The results of this study had a significance for the diagnosis as well as understanding of endometriosis.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2008年第3期168-173,共6页
Reproduction and Contraception
基金
上海市医学重点学科建设项目(05III016)
