摘要
目的:通过测定鼻息肉中核因子κB亚单位p50(NF-κB-p50)的活性及其与IL-4、γ-IFN表达的关系,来探讨p50对Th1/Th2细胞因子表达调节的可能机制。方法:采用SP免疫组织化学染色方法检测40例鼻息肉患者和20例正常钩突黏膜中IL-4、γ-IFN、p50的表达,以核染阳性率来评估p50活性。对p50活性与IL-4、γ-IFN因子进行直线相关分析。结果:①鼻息肉中p50细胞质、细胞核均有阳性表达,主要位于上皮细胞、炎症细胞及腺上皮细胞细胞质,p50活性显著增强(P<0.01),IL-4表达亦明显增强(P<0.01),γ-IFN表达无明显改变(P>0.05);②Pearson相关性分析,发现p50活性与IL-4表达呈直线正相关关系,r=0.70(P<0.01),而与γ-IFN表达无相关性,r=0.14(P>0.05)。结论:鼻息肉中p50的活性明显增强,p50的激活上调IL-4的表达可能是鼻息肉中Th1/Th2因子失衡的重要环节之一。
Objective：To explore the possible regulation mechanism of nuclear factor-kappa B subunit p50 on Th1/Th2 cytokine in nasal polyps. Method：The expression of IL-4 ,γ-IFN and p50 were examined by the method of immunohistochemistry staining in 40 cases of nasal polyps and 20 normal subjects, and the linear correlations between activity of p50 and IL-4,γ-IFN were evaluated. Result： The expression of p50 mainly distribute in the cytoplasm of epithelia, inflammatory cell and the epithelia of gland. The expression of IL-4 and the activity of p50 was increased significantly in nasal polyps（all P 〈0.01）. But the expression of γ-IFN had no significant difference between the groups（ P〉0.05）. Pearson correlation analysis demonstrated that p50 nuclear-presented closely correlation with IL-4 level, but not γ-IFN. The correlation coefficient was 0. 70 for p50 and IL-4（ P 〈0.01 ）,0.14 for p50 and γ- IFN（ P 〉0.05 ）. Conclusion：The activity of p50 in nasal polyps is increased significantly. The activiation of p50 may play an important role in the imbalance of Th1/Th2-typed cytokines by upregulating the leval of IL-4.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第5期211-214,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
