摘要
建立了犬血浆和小鼠组织、尿和粪中紫杉醇的ＨＰＬＣ检测法。生物样品用乙醚提取后在Ｃ１８柱上以０．０１ｍｏｌ／Ｌ磷酸二氢钠－甲醇（４０６０，ｐＨ＝４．３）为流动相，安定为内标，检测波长为２３３ｎｍ条件下进行检测。线性范围为１８．７～３６００μｇ／Ｌ，最低检测浓度为１０μｇ／Ｌ。紫杉醇在犬血浆和小鼠肝、尿、粪中的回收率分别为８６．０４％～９１．７３％，８７．６２％～９２．１２％，８６．７８％～９２．１６％，７９．８３％～８５．６２％；日内ＲＳＤ分别为２．３３％～３．７８％，１．３７％～４．５５％，３．５４％～４．２８％，２．３０％～４．０６％；日间ＲＳＤ分别为３．３１％～４．３８％，４．７２％～５．５１％，６．７５％～８．５３％，４．４３％～６．６７％。用本法对家犬ｉｖ紫杉醇后的药代动力学进行了研究，并报告了有关的动力学参数。
A HPLC method was developed for the determination of taxol in dog plasma and mouse tissue,urine and feces.All samples were extracted with diethyl ether and separated on a C 18 column with the mobile phase consisted of 0 01 mol/L NaH 2PO 4 buffer-methanol(40∶60,pH=4 3).The internal standard was diazepam.The detector was set at UV 233nm.The linear range of the calibration curve was 18 7～3600 μg/L and the minimal detectable concentration was 10μg/L.The recoveries of taxol in dog plasma and mouse liver,urine,feces were 86 04%～91 73%,87 62%～92 12%,86 78%～92 16%,79 83%～85 62%,respectively.The RSDs of within-day were 2 33%～3 78%,1 37%～4 55%,3 54%～4 28%,2 30%～4 06%,and RSDs of day-to-day were 3 31%～4 38%,4 72%～5 51%,6 75%～8 53%,4 43%～6 67%,respectively. The method has been used in pharmacokinetic studies of taxol in dogs after iv 3 0 mg/kg and their pharmacokinetic parameters were reported.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1997年第5期301-304,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
紫杉醇
高效液相色谱
血药浓度
taxol,HPLC,plasma concentration,pharmacokinetics
