摘要
在硼酸缓冲液和In(Ⅲ)-HQS荧光络合体系中加入一定浓度的半胱氨酸,体系的荧光强度与半胱氨酸的加入量成正比,从而提出一种新的荧光光度法测定半胱氨酸。该方法测定半胱氨酸的检出限为15ng·ml-1(SNR=3),大多数的氨基酸即使浓度较高时也无干扰。用该方法测定胱氨酸电解液中的半胱氨酸回收率为95.7%～101.3%。
The addition of cysteine （Cys） to the fluorescence system of In （ Ⅲ ）8-hydroxyquinoline-5- sulphonic acid complex （In （ Ⅲ ）-HQS） in H3BO3-Na2B4O7 buffer （pH 8.50） solution led to immediate fluorescence inhibition, which was proportional to their amounts. Based on this finding,a new spectrofluorimetric method for the determination of Cys has been developed. The detection limits was 15ng·mL^-1（ SNR =3）. Most amino acids had no interference at high concentrations. The proposed method has been applied to the determination of Cys in cysteine electrolyte, with recoveries of 95.7%～101.3%.
出处
《广西科学》
CAS
2008年第1期60-63,共4页
Guangxi Sciences
